Time-Course Evaluation of Oxidative Stress-Related Biomarkers after Renal Transplantation

Figures & data

Table 1. Characteristics of the study population.

Patients	39
Sex (M/F)	23/16
Age of recipients (years)	57 ± 10
BMI (kg/m^2)	26.7 ± 4.5
Dialysis before KT (months)	22.2 ± 27.7
Age of donors (years)	41.9 ± 14.4
Etiology of chronic renal failure
Chronic glomerulonephritis	13 (33.3%)
Chronic tubulo-interstitial nephritis	5 (12.8%)
Autosomal dominant polycystic kidney disease	4 (10.3%)
Diabetic nephropathy	7 (17.9%)
Nephrosclerosis	5 (12.8%)
Unknown cause	5 (12.8%)

Figure 1. The plasma creatinine level (A) and glomerular filtration (B) during posttransplantation period. The data are expressed as differences of the value after KT (days 1, 7, 30, 90, and 180) and the value before KT (day 0).

Table 2. Hematological and biochemical biomarkers before and after renal transplantation.

Parameter	Units	Before KT	Day 1	Day 7	Day 30	Day 90	Day 180
Erythrocytes	10^12/L	3.58 (2.43; 4.61)	3.12 (2.27; 4.24)	3.07 (2.31; 3.85)	3.64 (2.68; 4.62)	4.29 (3.19; 5.72)	4.58 (3.04; 6.03)
Hematocrit		0.34 (0.20; 0.40)	0.30 (0.20; 0.30)	0.29 (0.20; 0.30)	0.34 (0.30; 0.40)	0.40 (0.30; 0.50)	0.41 (0.30; 0.50)
Hemoglobin	g/L	114 (80; 146)	100 (79; 130)	96 (78; 120)	114 (85; 143)	133 (107; 183)	140 (81; 172)
Total protein	g/L	73.5 (59.1; 92.5)	59.0 (34.3; 69.9)	57.9 (44.9; 77.1)	67.5 (48.1; 88.4)	69.5 (57.3; 78.6)	70.4 (59.6; 79.3)
Albumin	g/L	45.0 (38.0; 55.0)	36.0 (26.0; 42.0)	34.0 (25.0; 41.0)	42.0 (29.0; 50.0)	44.0 (35.0; 52.0)	45.0 (37.0; 51.0)
C-reactive protein	mg/L	3.0 (1; 27)	19.0 (3.0; 86.0)	10.0 (1.0; 155.0)	2.0 (1.0; 90.0)	2.0 (1.0; 129.0)	1.5(1.0; 27.0)
HDL-cholesterol	mmol/L	1.19 (0.6; 2.4)	1.24 (0.70; 2.50)	0.88 (0.50; 2.70)	1.42 (0.80; 2.80)	1.76 (0.70; 3.70)	1.55 (0.60; 3.80)
LDL-cholesterol	mmol/L	2.64 (0.61; 5.39)	2.31 (0.39; 5.04)	2.33 (0.48; 3.87)	3.30 (1.58; 6.98)	2.75 (0.67; 6.77)	3.01 (1.50; 4.90)
Creatinine	μmol/L	666 (343; 969)	430 (158; 790)	275 (69; 988)	116 (80; 436)	106 (76; 160)	108 (72; 148)
Glomerular filtration	mL/s	0.14 (0.07; 0.29)	0.22 (0.13; 0.58)	0.27 (0.08; 1.42)	0.90 (0.15; 1.36)	1.02 (0.56; 1.62)	0.98 (0.61; 1.76)
Uric acid	μmol/L	313 (125; 901)	257 (115; 748)	437 (132; 682)	330 (153; 865)	325 (177; 644)	349 (169; 546)
Urea	mmol/L	17.8 (6.4; 30.6)	11.8 (7.0; 18.4)	26.4 (6.2; 48.9)	8.7 (4.3; 63.0)	8.0 (4.7; 13.1)	7.2 (3.9;11.3)
Ca	mmol/L	2.3 (1.9; 2.6)	2.1 (1.7; 2.5)	2.1 (1.7; 2.7)	2.3 (1.6; 2.7)	2.4 (2.0; 2.8)	2.4 (1.8; 2.9)

Notes: LDL, low-density lipoprotein; HDL, high-density lipoprotein. The values are expressed as median (first quartile; third quartile).

*p < 0.05 versus day 0.

Figure 2. The long-term alteration of selected parameters of oxidative stress: advanced oxidation protein products (A), total antioxidant capacity (B), erythrocyte lipid peroxidation (C), and erythrocyte reduced glutathione (D) during posttransplantation period. The data are expressed as differences of the value after KT (days 1, 7, 30, 90, and 180) and the value before KT (day 0).

Table 3. Oxidative stress biomarkers before and after renal transplantation.

	Units	Before KT	Day 1	Day 7	Day 30	Day 90	Day 180
AOPP	μmol/L	173.1 (58.4; 592.5)	66.4 (0; 244.8)	101.7 (12.3; 489.5)	132.3 (32.6; 784.0)	139.3 (17.1; 404.9)	120.0 (42.1; 312.0)
TAC	mmol/L	1.93 (0.62; 2.76)	1.54 (0.91; 2.21)	1.73 (0.67; 2.35)	1.73 (1.24; 2.43)	1.60 (1.22; 2.50)	1.72 (1.29; 2.17)
TBARS^a	nmol/g	0.42 (0.16; 0.94)	0.40 (0.17; 0.83)	0.37 (0.23; 0.75)	0.38 (0.18; 0.78)	0.37 (0.23; 0.54)	0.36 (0.22; 0.72)
GSH^a	μmol/g	7.69 (1.91; 23.27)	8.67 (0.60; 23.00)	9.90 (0.96; 23.01)	9.31 (5.67; 27.90)	11.06 (6.49; 32.70)	11.59 (1.04; 31.10)
SOD^a	U/g	2.43 (0.65; 5.25)	2.48 (0.46; 6.54)	2.46 (0.07; 5.99)	2.58 (0.27; 6.52)	2.53 (0.49; 5.11)	2.37 (0.30; 5.53)
CAT^a	μmol/min/g	73.0 (39.0; 167.8)	64.0 (32.7; 133.8)	63.9 (0.50; 129.5)	65.9 (31.9;152.0)	69.6 (26.4; 168.3)	68.7 (35.0;137.9)
GPX^a	μmol/min/g	23.0 (11.0; 58.4)	23.1 (9.6; 54.2)	23.4 (10.8; 53.3)	22.6 (10.0; 54.6)	23.6 (7.0; 65.7)	22.1 (7.0; 54.2)

Notes: AOPP, advanced oxidation protein products; TAC, total antioxidant capacity; TBARS, thiobarbituric acid-reactive substances; GSH, glutathione; SOD, superoxide dismutase; CAT, catalase; GPX, glutathione peroxidase. The values are expressed as median (first quartile; third quartile).

^aThe value is expressed in grams of hemoglobin.

*p < 0.05 versus day 0.