The Role of Angiotensin II Receptor 1 (AT1) Blockade in Cisplatin-Induced Nephrotoxicity in Rats: Gender-Related Differences

Figures & data

Figure 1.  (A) and (B) The serum levels of BUN and Cr in male and female animals, respectively, treated with losartan + CP, CP (positive control group), and saline (negative control group). (C) The percentage weight changes in male and female animals, respectively. The percentage weight change was calculated as [(last day weight – weight on the CP injection day)/weight on the CP injection day] × 100. The asterisk (*) indicates significant difference (p < 0.05) when compared with the negative control group. The sign (#) indicates significant difference (p < 0.05) when compared with the positive control group.

Table 1.  The serum levels of Mg, TP, and nitrite in both genders.

		Gender
		Male			Female
Parameter	Group	Mg	TP (g/dL)	Nitrite (μmol/L)	Mg (mg/dL)	TP (g/dL)	Nitrite (μmol/L)
	L + CP	3.73 ± 0.50	5.6 ± 0.6	15.61 ± 5.6	3.36 ± 0.33	6.32 ± 0.28	15.26 ± 3.7
	CP	3.69 ± 0.44	5.3 ± 0.3	22.37 ± 5.9	3.22 ± 0.37	6.23 ± 0.32	12.78 ± 3.9
	C	3.00 ± 0.13	6.4 ± 0.3	13.71 ± 5.8	2.66 ± 0.19	6.65 ± 0.13	25.04 ± 15.5
p		0.19	0.17	0.56	0.06	0.80	0.31

Notes: No significant differences were observed in serum levels of TP, Mg, and nitrite between the groups of both genders. L, losartan; CP, cisplatin; C, negative control (saline); Mg, magnesium; TP, total proteins.

Figure 2.  (A) and (B) Total kidney weight/100 g of body weight values and KTDSs in male and female animals, respectively, treated with losartan + CP, CP (positive control group), and saline (negative control group). The asterisk (*) indicates significant difference (p < 0.05) in comparison with the negative control group. The sign (#) indicates significant difference (p < 0.05) in comparison with the positive control group. (C) Correlations between total kidney weight/100 g of body weight and KTDS in male and female animals, respectively. Significant correlations (p < 0.05) were observed between KTDS and kidney weight in both genders.

Figure 3.  The images (magnification × 100) of the kidney tissue. (A) group 1: male, losartan + CP; (B) group 2: female, losartan + CP; (C) group 3: male, CP; (D) group 4: female, CP; (E) group 5: male, saline; and (F) group 6: female, saline. More tissue damage was observed in female losartan + CP and male CP-treated groups.