Caffeic Acid Phenethyl Ester Protects Kidneys against Acetylsalicylic Acid Toxicity in Rats

Figures & data

Table 1. Oxidant and antioxidant parameters in rat groups (mean ± standard deviation).

	Kidney			Serum
	PON-1	TAC	TOS	PON-1	TAC	TOS
Groups	(U/mg protein)	(mmol Trolox Eq/g protein)	(mmolH2O2 Eq/g protein)	(U/mg protein)	(mmol Trolox Eq/g protein)	(mmolH2O2 Eq/g protein)
Control (1)	6.30 ± 2.52	1.14 ± 0.27	46.73 ± 21.16	171.58 ± 32.36	1.14 ± 0.16	57.14 ± 12.54
ASA 50 (2)	5.34 ± 2.42	0.90 ± 0.23	73.84 ± 24.88	132.93 ± 22.43	0.83 ± 0.07	153.67 ± 30.15
ASA 50 + CAPE (3)	5.80 ± 2.14	0.93 ± 0.24	73.05 ± 34.93	160.07 ± 21.98	1.37 ± 0.26	79.82 ± 21.33
ASA 100 (4)	2.89 ± 1.17	0.73 ± 0.27	103.10 ± 31.66	126.00 ± 13.01	0.82 ± 0.07	161.19 ± 14.17
ASA 100 + CAPE (5)	6.44 ± 1.64	1.04 ± 0.23	107.11 ± 73.89	147.87 ± 17.32	1.17 ± 0.27	66.23 ± 12.28
p-Value between groups
1–2	0.462	0.093	0.036	0.036	0.001	0.001
1–4	0.003	0.014	0.005	0.016	0.001	0.001
2–3	0.401	0.834	0.834	0.009	0.001	0.002
4–5	0.002	0.046	0.529	0.016	0.001	0.001

Notes: ASA, aspirin; CAPE, caffeic acid phenethyl ester; PON-1, paraoxanase-1; TAC, total antioxidant capacity; TOS, total oxidant status.

1: control group, 2: ASA 50 treatment group, 3: ASA 50 + CAPE treatment group, 4: ASA 100 treatment group 5: ASA 100 + CAPE treatment group.

Figure 1. (A) Control group. Renal tubular structures (H&E, ×400). (B) ASA 50 mg group. Moderate cell swelling (arrow), some nuclear loss (arrowhead), and degenerative changes in tubular structures (H&E, ×400). (C) ASA 50 mg + CAPE group. Mild tubular cell swelling, some nuclear loss, and minimal degenerative changes in the tubules (H&E, ×400). (D) ASA 100 mg group. Prominent degenerative changes with cell swelling (arrows), much nuclear loss (arrowheads), nuclear pyknosis in the tubular cells, and moderate architectural impairment in the tubular structures (H&E, ×400). (E). ASA 100 mg + CAPE group. Improvement in the tubular structures, reduction of nuclear loss, and minimal cellular swelling compared with the ASA 100 mg group (Figure 1D) (H&E, ×400).