Association between Endothelial Nitric Oxide Synthase Glu298Asp Gene Polymorphism and Diabetic Nephropathy Susceptibility

Figures & data

Figure 1.  Flowchart of our studies.

Table 1.  Characteristics of the studies evaluating the effects of eNOS Glu298Asp gene polymorphism on DN risk.

		DN				Control				Tallele (%)
First author, year	Ethnicity	TT	TG	GG	Total	TT	TG	GG	Total	DN	Control
Noiri et al. (2002)	African			49	72			251	307
Nagase et al. (2003)	Asian	2	8	38	48	2	18	250	270	12.5	4.07
Shin et al. (2004)	Asian	0	23	93	116	0	7	52	59	9.91	5.93
Fu et al. (2006)	Asian	0	27	32	59	0	12	49	61	22.88	9.84
Thaha et al. (2008)	Asian	1	31	7	39	2	26	72	100	42.31	15
El-Din and Hamdy (2011)	Caucasian	12	18	10	40	1	7	12	20	52.5	22.5
Santos et al. (2011)	Brazil	32	166	176	374	22	95	118	235	30.75	29.57
Li1 et al. (2011)	Asian	0	17	85	102	1	33	171	205	8.33	8.54

Figure 2.  The pooled OR indicated that T allele was associated with DN risk in overall populations.

Figure 3.  The pooled OR indicated that GG genotype was associated with DN risk in overall populations.

Table 2.  Meta-analysis of the association of eNOS Glu298Asp gene polymorphism with the risk of DN.

Genetic contrasts	Group and studies subgroups		Q-test p-value	Model selected	OR (95% CI)	p
T versus G	Overall	7	0.0001	Random	2.13 (1.26, 3.61)	0.005
	Asian	5	0.01	Random	2.30 (1.31, 4.07)	0.004
	Caucasian	1	–	Fixed	3.81 (1.61, 9.02)	0.002
	Brazil	1	–	Fixed	1.06 (0.82, 1.36)	0.66
TT versus TG + GG	Overall	7	0.15	Fixed	1.25 (0.76, 2.06)	0.38
	Asian	5	0.43	Fixed	2.04 (0.54, 7.71)	0.29
	Caucasian	1	–	Fixed	8.14 (0.986, 7.94)	0.05
	Brazil	1	–	Fixed	0.91 (0.51, 1.60)	0.73
GG versus TG + TT	Overall	8	0.0001	Random	0.40 (0.23, 0.69)	0.0009
	Asian	5	0.0005	Random	0. 34 (0.15, 0.77)	0.01
	Caucasian	1	–	Fixed	0.22 (0.07,0.70)	0.01
	African	1	–	Fixed	0.45 (0.25, 0.80)	0.007
	Brazil	1	–	Fixed	0.88 (0.64, 1.22)	0.45
Sensitivity analysis
T versus G	Overall	6	0.0001	Random	1.99 (1.13, 3.49)	0.02
	Asian	4	0.008	Random	2.10 (1.06, 4.19)	0.03
	Caucasian	1	–	Fixed	3.81 (1.61, 9.02)	0.002
	Brazil	1	–	Fixed	1.06 (0.82, 1.36)	0.66
TT versus TG + GG	Overall	6	0.25	Fixed	1.16 (0.69, 1.93)	0.58
	Asian	4	0.75	Fixed	0.99 (0.14, 6.79)	0.99
	Caucasian	1	–	Fixed	8.14 (0.98, 67.94)	0.05
	Brazil	1	–	Fixed	0.91 (0.51, 1.60)	0.73
GG versus TG + TT	Overall	6	0.00001	Random	0.40 (0.19, 0.82)	0.01
	Asian	4	0.0002	Random	0.35 (0.12, 0.99)	0.05
	Caucasian	1	–	Fixed	0.22 (0.07, 0.70)	0.01
	Brazil	1	–	Fixed	0.88 (0.64, 1.22)	0.45