Aged Rats Are Susceptible to Nephrotoxicity Induced by Iodinated Contrast Media

Figures & data

Table 1.  Changes of Scr and Ccr in different groups.

					Scr (μmol/L)		Ccr (ml/min per 100 g body weight)
Age	Group	n	Body weight (g)	TC (mmol/L)	Baseline	After 48 h	Baseline	After 48 h
12 months	AdNS	6	535.9 ± 59.01	1.49 ± 0.22	29.32 ± 1.74	30.02 ± 1.69	0.44 ± 0.029	0.43 ± 0.027
	AdCM	6	532.8 ± 67.14	1.51 ± 0.21	30.14 ± 2.06	35.28 ± 2.02^a,^b	0.43 ± 0.028	0.41 ± 0.022^a,^b
24 months	AgNS	6	609.6 ± 78.56	2.31 ± 0.18^b	31.27 ± 1.95	33.53 ± 1.92	0.41 ± 0.023	0.42 ± 0.022
	AgCM	6	588.4 ± 70.15	2.36 ± 0.23^b,^d	31.52 ± 4.51	43.33 ± 4.52^a,^c,^d	0.42 ± 0.033	0.38 ± 0.031^a,^c,^d

Notes: Data are mean ± SD () for each group. TC, serum total cholesterol; Scr, serum creatinine; Ccr, creatinine clearance rate; AdNS, adult saline control group; AdCM, adult contrast media group; AgNS, aged saline control group; AgCM, aged contrast media group.

^ap < 0.05, compared with baseline.

^bp < 0.05, compared with AdNS group.

^cp < 0.05, compared with AgNS group.

^dp < 0.05, compared with AdCM group.

Table 2.  Comparison of intrarenal ACE and Ag II activity in different groups.

Age	Group	n	ACE (U/mg protein)	Ag II (ng/g protein)
12 months	AdNS	6	3.78 ± 1.12	2576.18 ± 406.76
	AdCM	6	4.09 ± 1.65	3165.50 ± 286.15
24 months	AgNS	6	2.62 ± 0.48^a	2132.1 ± 208.29^a
	AgCM	6	7.82 ± 1.94^b,^c	5302.72 ± 402.40^b,^c

Notes: All values of enzyme activity are presented as mean ± SD (). ACE, angiotensin-converting enzyme; Ag II, angiotensin II; AdNS, adult saline control group; AdCM, adult contrast media group; AgNS, aged saline control group; AgCM, aged contrast media group.

^ap < 0.05, compared with AdNS group.

^bp < 0.01, compared with AgNS group.

^cp < 0.01, compared with AdCM group.

Table 3.  Comparison of intrarenal ROS-related parameters in different groups.

Age	Group	n	MDA (nmol/mg protein)	SOD (U/mg protein)	NADPH (U/mg protein)
12 months	AdNS	6	3.34 ± 0.90	466.71 ± 45.72	21.8 ± 3.37
	AdCM	6	3.96 ± 1.14	429 ± 26.29	27.9 ± 3.41
24 months	AgNS	6	5.52 ± 1.42^a	439.23 ± 37.29^a	28.72 ± 3.49^a
	AgCM	6	7.66 ± 2.01^b,^c	412.05 ± 41.30^b	96.48 ± 13.68^b,^c

Notes: All values of enzyme activity are presented as mean ± SD (). MDA, malondialdehyde; SOD, superoxide dismutase; NADPH, reduced form of nicotinamide adenine dinucleotide phosphate oxidase; AdNS, adult saline control group; AdCM, adult contrast media group; AgNS, aged saline control group; AgCM, aged contrast media group.

^ap < 0.05, compared with AdNS group.

^bp < 0.01, compared with AgNS group.

^cp < 0.01, compared with AdCM group.

Figure 1.  Representative renal histomorphological changes. (A) AdNS, adult saline control group. (B) AdCM, adult contrast media group. (C) AgNS, aged saline control group. (D) AgCM, aged contrast media group (HE staining, original magnification ×200).

Figure 2.  The scores of tubular injury. AdNS, adult saline control group; AdCM, adult contrast media group; AgNS, aged saline control group; AgCM, aged contrast media group.

Notes: ^ap < 0.01, compared with AgNS group.^bp < 0.01, compared with AdCM group.^cp < 0.01, compared with AdNS group.