Circulating purine compounds, uric acid, and xanthine oxidase/dehydrogenase relationship in essential hypertension and end stage renal disease

Figures & data

Table 1. Plasma urea, creatinine, and blood pressure in the investigated groups.

Investigated parameters	Control20	Essential hypertension30	Dialysis28
Plasma U urea  (mmol/L)	4.09 ± 1.35	6.06 ± 2.08	22.48 ± 7.43*** ^ooo
Plasma creatinine  (µmol/L)	72.06 ± 5.18	84.37 ± 13.97	597.03 ± 101.88*** ^ooo
Systolic TA  (mmHg)	134.45 ± 12.44	163.18 ± 24.56	150.34 ± 23.28
Dyastolic TA  (mmHg)	79.98 ± 9.99	94.65 ± 17.36	89.99 ± 20.98

***p < 0.001, statistical significance compared with the control.

^ooop < 0.001, statistical significance compared with the patients with essential hypertension.

Figure 1. The concentration of circulating blood nucleotides, ATP, ADP, AMP, and uric acid in investigated groups. For the evaluation of purine nucleotides concentration, fresh blood was immediately processed for their isolation after adding stabilizing solution of retard ATP degradation. ***p < 0.001 compared with the control; *p < 0.05 compared with the control; ^ooop < 0.001 compared with the essential hypertension group.

Figure 2. The index of ATP/ADP and ATP/uric acid in investigated groups. For the evaluation of purine nucleotides concentration, fresh blood was immediately processed for their isolation after adding stabilizing solution o retard ATP degradation. ***p < 0.001 compared with the control; ^ooop < 0.001 compared with the essential hypertension group.

Figure 3. The activities of total Xanthine oxidoreductase (XOD), xanthine oxidase (XO), and xanthine dehydrogenase (XDH) in plasma of investigated groups. Xanthine oxidoreductase (XOD) and xanthine oxidase (XOD) were measured in plasma according to the liberation of uric acid,³⁴ in the presence of NADH (XOD) or absence of NADH (XO) when only molecular oxygen was electron acceptor. ***p < 0.001 compared with the control; *p < 0.05 compared with the control; ^ooop < 0.001 compared with the essential hypertension group.

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