Immunohistochemical study of tubular epithelial cells and vascular endothelial cells in glomerulonephritis

Figures & data

Figure 1. Intense positive vimentin immunostaining at the level of the interstitium, peritubular, and periglomerular. Vimentin stain LSAB2-DAB × 100.

Figure 2. Positive αSMA immunostaining in the cytoplasm and nuclei of tubular epithelial structures. αSMA stain LSAB2-DAB × 200.

Figure 3. Interstitial capillary and arteriolar endothelium positive for TGFβ stain. TGFβ stain LSAB2-DAB × 200.

Table 1. Correlation between immunohistochemical staining scores at the level of tubular epithelial cells (TEC) and interstitial histological scores and clinical data (prox, proximal; dist, distal).

Pearson correlation	Interstitial edema	Interstitial infiltrate	Interstitial fibrosis	Tubular atrophies	Proteinuria	Serum creatinine	eGFR
αSMA prox. TEC
r	0.21009156	−0.08227098	0.03898	0.0850556	−0.0757	−0.12323	0.221282
p	0.14644391	0.34165263	0.42346	0.3365800	0.3566	0.27014	0.133666
αSMA dist. TEC
r	0.1994508	−0.16858008	−0.0587	−0.1302433	0.063622	−0.15351	0.32015
p	0.1592839	0.20029769	0.38548	0.2586517	0.378748	0.222297	0.051761
Vimentin prox. TEC
r	0.099812798	0.313162826	0.25943603	0.06529312	−0.2786578	0.21132465	−0.34791524
p	0.275512236	0.02778171	0.04279996	0.34846625	0.0474500	0.10139955	0.01616026
Vimentin dist. TEC
r	0.256134317	0.223258053	0.05042303	−0.00074431	−0.14634197	0.32781289	−0.36793017
p	0.060307715	0.088932234	0.38184585	0.49823070	0.19371777	0.02225947	0.01152254
TGFβ prox. TEC
r	−0.189164364	−0.172968478	−0.1383094	−0.1537955	−0.3027728	−0.02682417	0.01553297
p	0.118104471	0.139745243	0.19423911	0.16851610	0.02878059	0.43389092	0.46160562
TGFβ dist. TEC
r	−0.083709401	−0.183802808	−0.1107815	−0.1665446	−0.23749043	0.05062329	−0.06327586
p	0.301413153	0.125000099	0.2452423	0.14900329	0.07002606	0.37663731	0.34714952

Table 2. Correlation between immunohistochemical scores for vascular endothelial cells (VEC) staining and interstitial histological scores and clinical data.

	Interstitial edema	Interstitial infiltrate	Interstitial fibrosis	Tubular atrophies	Proteinuria	Serum creatinine	eGFR
Vimentin VEC
r	−0.199299803	−0.329175908	−0.3469841	−0.4079876	−0.05671652	−0.17901234	0.20386943
p	0.118485114	0.023328144	0.01768722	0.0060939	0.3712464	0.14454750	0.11308439
TGFβ VEC
r	0.203251664	0.17368135	0.19315847	0.16755051	−0.25851234	0.10722386	−0.23323048
p	0.101234127	0.138741544	0.1131387	0.14752826	0.0436287	0.2523020	0.07111387