Role of CD2-associated protein in podocyte apoptosis and proteinuria induced by angiotensin II

Figures & data

Table 1. CD2AP mRNA levels at different time.

	The control	Ang II	Losartan
8 h	0.86 ± 0.02	0.87 ± 0.01	0.86 ± 0.01
24 h	0.91 ± 0.01	0.52 ± 0.03^#	0.90 ± 0.04*
48 h	1.04 ± 0.03	0.40 ± 0.02^#	0.98 ± 0.03*

Note: In the control, Ang II and Losartan group, CD2AP mRNA expression had no changes at 8 h (p > 0.05), incubation of the podocytes with Ang II markedly decreased CD2AP mRNA levels at 24 and 48 h (^#p < 0.05). In contrast, Losartan completely prevented the mRNA decrease in CD2AP induced by Ang II (*p < 0.05).

Figure 1. Western blot band of CD2AP of control group, Ang II group and Losartan group at different time points. (a): The control, (b): AngII, (c): Losartan.

Table 2. CD2AP protein levels at different time.

	The control	Ang II	Losartan
8 h	0.061 ± 0.010	0.060 ± 0.011	0.059 ± 0.010
24 h	0.084 ± 0.011	0.068 ± 0.013	0.076 ± 0.010*
48 h	0.092 ± 0.012	0.040 ± 0.010	0.085 ± 0.011*

Note: CD2AP protein levels decreased at 24 h and 48 h in the cells that were exposed to Ang II without Losartan, whereas cells that were treated with Losartan were resistant to Ang II-induced CD2AP protein levels decreased. *p < 0.05 versus Losartan (–) Ang II (+).

Figure 2. The percentage of apoptosis measured by Annexin-V FITC and PI staining. PI and Annexin V apoptosis kit by double staining was used to detect the apoptosis rate and subjected to flow cytometric analysis, in which X axis is Annexin V(+) and Y axis is PI (+), Q1 is PI (+), Annexin (−V); Q2 is PI (+), Annexin (+V), the late apoptosis or dead cells; Q3 is PI (−), Annexin V (−), the normal living cell; Q4 is Annexin V (+), PI (−), the early apoptosis cells. Losartan prevents Ang II-induced apoptosis. The percentage of apoptotic cells, measured by Annexin-V FITC and PI staining increased at 24 h in the cells that were exposed to Ang II without Losartan, whereas cells that were treated with Losartan were resistant to Ang II-induced apoptosis. *p < 0.05 versus Losartan (–) Ang II (+).