Treg cells in thrombotic thrombocytopenic purpura associated with systemic lupus erythematosus patients

Figures & data

Figure 1. Treg cells in peripheral blood by flow cytometry. Notes: Treg cells were counted using the indicated gates and are enumerated in Table 1. α: Treg cells, I: TTP with SLE patients, II: Control groups. Treg cells significantly decreased in TTP with SLE patients compared to the control Groups (p < 0.05).

Table 1. The number of Treg cells in peripheral blood (mean ± SD)%.

	TTP with SLE groups	Controls
Trg cells	0.95 ± 0.107^a	3.02 ± 0.214

Note: Treg cells significantly decreased in TTP with SLE patients compared to the control group (^ap < 0.05).

Table 2. Clinical and histopathological findings in TTP with SLE patients.

	TTP with SLE group	Control group
Proteinuria (g/day)	3.25 ± 0.61
Hematuria (×10^4cells/mL)	375.86 ± 5.49
BUN (mg/dL)	23.7 ± 0.68*	13.4 ± 0.87
Serum Cr (mg/dL)	8.73 ± 0.26*	0.69 ± 0.14
Serum uric acid (µmol/L)	458.73 ± 84.75*	237.48 ± 39.15
eGFR (mL/min/1.73 m^2)	78.14 ± 10.23*	105.32 ± 10.41
Serum C3 (mg/dL)	39.5 ± 17.5*	123.5 ± 12.8
Serum IL-2 (pg/mL)	113.7 ± 15.6*	23.5 ± 13.1
supernatant IL-4 (pg/mL)	524 ± 23.01*	201.57 ± 35.72
Serum IL-6 (pg/mL)	59.12 ± 11.32*	30.52 ± 10.31
AI (median)/CI (median)	10.5 ± 2.3/    3.5 ± 0.7
vWF(%)	207.71 ± 13.00
VCAM (ng/mL)	1226.47 ± 132.97
AECA (positive %)	71
ADAMTS13-antibodies (positive%)	85

Notes: AI, activity index; CI, chronicity index.

*p < 0.05 compared with control.

Table 3. Correlation between the frequency of Treg cells and clinical parameters in TTP with SLE patients.

	Treg cells (%)
	r	p
SBP	−0.248	n.s.
DBP	−0.320	n.s.
BUN	−0.513	<0.05
SUA	−0.527	<0.01
eGFR	0.859	<0.01
24-h UP	−0.574	<0.01
Hematuria	−0.215	n.s.
Serum C3	0.241	n.s.
Serum IL-2	−0.113	n.s.
Supernatant IL-4	−0.738	<0.01
Serum IL-6	−0.237	n.s.
vWF (%)	−0.226	n.s.
VCAM (ng/mL)	−0.176	n.s.

Notes: n.s., not significant; SBP, systolic blood pressure; DBP, diastolic blood pressure; Scr, serum creatinine; SUA, serum uric acid; eGFR, estimated glomerular filtration rate; 24-h UP, 24-hours urinary protein.