Compare the effects of intravenous and intraperitoneal mesenchymal stem cell transplantation on ultrafiltration failure in a rat model of chronic peritoneal dialysis

Figures & data

Figure 1. Study groups and experimental design.

Figure 2. H&E staining of representative samples of peritoneal tissues. (A) In UFF-C group, there was thickening of the submesothelial area with inflammatory cell infiltration and neoangiogenesis. (B) Control rat peritoneum. (C) In the MSC-IP group, there was submesothelial thickness similar compared to C group. (D) In the MSC-IV group, morphological changes did not significantly differ in from C group. (E) In the P group, increased submesothelial thickness and peritoneal fibrosis with neovascularization was seen (×10).

Table 1. Evaluation of peritoneal membrane histopathology and permeability of all groups.

	GROUPS
	Control n = 8	UFF-C n = 8	MSC-IP n = 9	MSC-IV n = 8	Placebo n = 9
Submesothelial thickness (µm)	66.3 ± 24.8	132.9 ± 40.1^a	99.0 ± 57.1^c	108.3 ± 44^c	208.2 ± 115^a,b,d,e
Inflammation
Increased	0	4^a	1	3	3^a
Normal	8	4	8	5	6
Neovascularization
Increased	1	6^a	2	3	6^a,d
Normal	7	2	7	5	3
Fibrosis
Increased	1	3	2	2	6^a,d,e
Normal	7	5	7	6	3
Mesothelial cells count
Decreased	1	6^a	3	4	7^a
Normal	7	2^a	6	4	2
UF (mL) median (min – max)	4 (−2 – 10)	−3 (−24 – 6)^a	7.8 (5 – 10)^b,c	3.3 (−16 – 14)^b	1 (−17 – 12)
D/Purea (mean ± SD)	0.40 ± 0.05	0.59 ± 0.14^a	0.57 ± 0.11^a	0.49 ± 0.04	0.58 ± 0.09^a
D/PCr (mean ± SD)	0.65 ± 0.07	0.88 ± 0.15^a	0.73 ± 0.15^b,c	0.77 ± 0.16	0.85 ± 0.12^a
D/Pprotein (mean ± SD)	114.8 ± 6.9	125.38 ± 37.5^a	119.4 ± 8.4	118.2 ± 15	129.9 ± 10.6^a
D/PNa (mean ± SD)	0.78 ± 0.02	0.84 ± 0.03^a	0.82 ± 0.03^a	0.77 ± 0.2	0.81 ± 0.03
D/D0glucose (mean ± SD)	1.45 ± 0.22	0.65 ± 0.35^a	1.31 ± 0.41^b,c	0.92 ± 0.04^a	0.90 ± 0.12^a
Glucose mass transfer (mean ± SE)	−9649 ± 7985.6	12005 ± 5911^a	−5170 ± 2759^b,c	5500 ± 1684^a,d	1752 ± 4006

^ap < 0.05, versus control group.

^bp < 0.05, versus UFF-C group.

^cp < 0.05, versus placebo group.

^dp < 0.05, versus MSC-IP group.

Figure 3. The immunofluorescence microscope images show the peritoneum slides, MSC-IP group (A) MSC-IV group and placebo group (C). GFP labeled-MSCs were observed along the mesothelium line in the MSC-IV and IP group (A) and (B) but were absent in the placebo group (B).

Table 2. Cytokines levels in the parietal peritoneum.

	GROUPS
	Control n = 8	UFF-C n = 8	MSC-IP n = 9	MSC-IV n = 8	Placebo n = 9
IL-6 (pg/mg protein) Mean ± SD	1080 ± 231	1473 ± 325	726 ± 274^a	1092 ± 376	1195 ± 285
TGF-β (pg/mg protein) Median (min – max)	882.7 (207–2126)	1811.1 (533–4107)	788 (68–1996)^b	1415 (487–3206)	4568 (341–7006)^c,d
VEGF (pg/mg protein) Mean ± SD	42 ± 20	39.8 ± 27.3	18.6 ± 7.3	22.5 ± 21	20.8 ± 15.6
TNF-α (pg/mg protein) Median (min – max)	69.4 (24–123)	105.9 (79.7–223.9)	40.4 (26.8–61.7)^a	52.08 (37.9–9.4)^a	63.4 (35.3–98.5)^a

^ap < 0.05 versus UFF-C group.

^bp < 0.05 versus placebo group.

^cp < 0.05 versus control group.

^dp < 0.05 versus MSC-IP group.