NPHS2 variation in Chinese southern infants with late steroid-resistant nephrotic syndrome

Figures & data

Table 1. Sequences of primers.

Exon	Forward primer	Reverse primer
1	AGCGACTCCACAGGGACTGC	CTGACGCCCCTTAGTTACCA
2	AGAATTGGACCAACAGATGC	AAGTGAGAATGGGCATGGTG
3	GCCCCCGCGGTCTTATGCCAAGGCCTTTTGAAGAC	GGGTTGAAGAAATTGGCAAGTCAGG
4	AAGGTGAAACCCAAACAGC	CGGTAGGTAGACCATGGAAA
5	AGGATTTACCACAGGATTAAGTTGTGCA	TAGCTATGAGCTCCCAAAGGGATGG
6	TATTTATAAATAAGGCACTGTGAAGTTAAATACAAC	CCCCCGCCCGCCAGAATATTTTCCTTTATCATACAG
7	GAGGCTTGCAAGTCTGTGTGAAAGC	AGGAAGCAAAGGGGAAATGTTCTCC
8	ATGCTCAGTGCTTGTCTGCT	TCACATTATGCCCCATCCTT

Figure 1. Direct-sequencing exon in NPHS2. The arrows indicate (A) NPHS2 288C > T heterozygous variation in exon 2; (B) 954T > C heterozygous in exon 8; (C) 1038A > G heterozygous in exon 8.

Table 2. The clinical and NPHS2 variations of 22 patients with SNPs.

Patient	Gender	Age at onset of INS (years)	Period of observation (years)	Time until late resistance from onset of INS (months)	Relapses until late resistance	Cytostatic drugs	Variation
1	F	0.2	1.0	2.0	12	CsA	288C > T(h)
							954T > C(h)
							1038A > G(h)
2	M	1.1	1.6	2.3	10	CsA/ Cph	288C > T(h)
							954T > C(h)
							1038A > G(h)
3	M	1.3	0.4	13	2	Cph	954T > C(h)
4	F	2.5	1.1	5.2	14	CsA/ Cph/	288C > T(h)
						CsA	954T > C(h)
							1038A > G(h)
5	F	0.6	1.3	4.2	5	Cph	954T > C(H)
6	M	0.8	0.7	5.9	6	CsA	954T > C(h)
							288C > T(h)
7	M	0.3	2.0	4.2	2	CsA	954T > C(H)
8	M	1.6	1.7	8.0	4	CsA	954T > C(h)
9	F	1.4	2.2	4.5	7	CsA	954T > C(h)
							1038A > G(h)
10	M	1.2	0.8	6.2	4	Cph	954T > C(h)
11	M	0.9	0.5	4.0	3	Cph	954T > C(h)

F, female; M, male; PNS, primary nephrotic syndrome; CsA, cyclosporin A; Cph, cyclophosphamide; h, heterozygous; H, homozygous.