Urine matrix metalloproteinases and their extracellular inducer EMMPRIN in children with chronic kidney disease

Figures & data

Table 1. Patient characteristics.

	Median values (lower – upper quartile) of analyzed parameters
Parameter	Control group (n = 23)	CKD (n = 41)
Age [years]	10.5 (5.0–14.5)	11.0 (4.0–17.0)
Gender	13 girls; 10 boys	17 girls; 24 boys
eGFR [mL/min]	105.0 (97.0–112.3)*	26.0 (16.8–38.0)
Urea [mg/dL]	32.0 (25.5–37.0)*	77.0 (55.0–94.5)
Albumin [g/dL]	–	4.3 (3.8–4.5)
Hemoglobin [g/dL]	12.8 (11.7–13.9)*	11.2 (10.5–12.2)
Parathormone [pg/mL]	–	125.0 (46.1–223.0)
hsCRP [mg/L]	0.5 (0.24–1.34)	0.6 (0.18–1.37)
Proteinuria [g/L]	0.01 (0–0.1)*	0.4 (0.03–0.6)
Urine creatinine [mg/dL]	114.0 (100.0–126.0)*	90.0 (72.0–110.0)

Mann–Whitney U test: *p < 0.001 control group versus CKD.

Table 2. The concentrations of analyzed parameters in serum of CKD children and controls.

Parameters	Median values (lower – upper quartile)
in serum	Control group (n = 23)	CKD (n = 41)
MMP-2 [ng/mL]	94.53 (88.88–115.77)*	159.45 (156.05–167.92)
MMP-7 [ng/mL]	2.23 (2.17–2.91)*	2.97 (2.27–3.05)
MMP-9 [ng/mL]	94.30 (91.00–100.80)*	415.60 (397.90–427.60)
EMMPRIN [pg/mL]	871.93 (854.86–906.07)*	1175.03 (1150.55–1211.54)
TGFbeta1 [ng/mL]	1221.99 (1195.0–1242.9)*	1738.88 (1717.61–1760.18)

Mann–Whitney U test: *p < 0.0001 control group versus CKD.

Table 3. The concentrations of analyzed parameters in urine of CKD children and controls.

Parameters	Median values (lower – upper quartile)
in urine	Control group (n = 23)	CKD (n = 41)
MMP-2 [ng/mg creat]	1.19 (1.03–1.43)*	2.64 (1.92–3.87)
MMP-7 [ng/mg creat]	0.009 (0.008–0.10)*	3.11 (2.71–3.91)
MMP-9 [ng/mg creat]	1.84 (1.63–2.12)*	4.49 (3.95–5.25)
EMMPRIN [pg/mg creat]	375.02 (313.30–402.44)*	1082.36 (1938.19–1361.49)
TGFbeta1 [ng/mg creat]	42.55 (38.44–47.97)*	258.55 (240.49–323.66)

Mann–Whitney U test: *p < 0.0001 control group versus CKD

Table 4. Correlations between examined parameters in serum and urine of CKD children.

Parameter	Serum MMP-2 [ng/mL]	Serum MMP-7 [ng/mL]	Serum MMP-9 [ng/mL]	Serum EMMPRIN [pg/mL]	Serum TGFbeta1 [ng/mL]
Urine MMP-2 [ng/mg creat]	p = 0.00006 R = −0.59	p = 0.0003 R = 0.53	p = 0.00003 R = 0.61	p = 0.41 R = 0.13	p = 0.50 R = −0.11
Urine MMP-7 [ng/mg creat]	p = 0.31 R = −0.16	p = 0.9 R = −0.02	p = 0.44 R = 0.13	p = 0.72 R = 0.06	p = 0.84 R = −0.03
Urine MMP-9 [ng/mg creat]	p = 0.039 R = −0.32	p = 0.16 R = 0.22	p = 0.048 R = 0.31	p = 0.21 R = 0.19	p = 0.76 R = −0.05
Urine EMMPRIN [pg/mg creat]	p = 0.49 R = −0.11	p = 0.94 R = −0.01	p = 0.64 R = 0.08	p = 0.69 R = −0.06	p = 0.91 R = 0.02
Urine TGFbeta1 [ng/mg creat]	p = 0.32 R = −0.16	p = 0.87 R = 0.03	p = 0.29 R = 0.17	p = 0.03 R = 0.27	p = 0.71 R = −0.06

R – Spearman's correlation coefficient.