L-Carnitine inhibits eryptosis induced by uremic serum and the related mechanisms

Figures & data

Figure 1. The effect of different mediums on PS, ROS and GSH of healthy erythrocytes. The arithmetic means ± standard error (mean ± SEM, n = 6) of PS, ROS and GSH following incubation for 24 and 48 h in Group C, U and L, respectively.

Figure 2. Original histogram of Annexin-V-binding of erythrocytes following exposure for 24 and 48 h to Group C, U and L. Blue area represents the quantity of Annexin-V-binding of erythrocytes. Fig.012 is Annexin-V-binding erythrocytes at 0 h. Fig.006, 014 and 004 are Annexin-V-binding erythrocytes at 24 h in Group C, U and L, respectively. Fig.015, 016 and 024 are Annexin-V-binding erythrocytes at 48 h in Group C, U and L, respectively.

Table 1. The results of PS, ROS, GSH in different group (mean ± SEM).

	PS		ROS		GSH
	24 h	48 h	24 h	48 h	24 h	48 h
Group C	3.43 ± 0.37	4.21 ± 0.44	14.83 ± 2.22	20.94 ± 1.78	31.27 ± 0.38	17.29 ± 0.54
Group U	6.51 ± 0.71^a,**	8.55 ± 0.76^a,**	33.12 ± 1.61^a,**	42.06 ± 1.81^a,**	25.66 ± 0.32^a,**	8.53 ± 0.59^a,**
Group L	5.80 ± 0.69^b,*	7.87 ± 0.76^b,*	26.29 ± 1.69^b,**	36.21 ± 2.00^b,**	27.54 ± 0.60^b,**	15.18 ± 0.42^b,**

*p < 0.05.

**p < 0.01.

^aGroup U versus Group C.

^bGroup L versus Group U.

Figure 3. Original histogram of ROS of erythrocytes following exposure for 24 and 48 h to Group C, U and L. Blue area represents the quantity of ROS. Fig.001 is ROS at 0 h. Fig.003, 014 and 007 are ROS at 24 h in Group C, U and L, respectively. Fig.023, 035 and 048 are ROS at 48 h in Group C, U and L, respectively.

Table 2. The correlation analysis of PS, ROS and GSH.

	PS and ROS	PS and GSH	ROS and GSH
24 h	0.87	−0.79	−0.83
p	0.000	0.000	0.000
48 h	0.92	−0.69	−0.80
p	0.000	0.000	0.000