Protective effects of probiotic Lactobacillus plantarum BJ0021 on liver and kidney oxidative stress and apoptosis induced by endosulfan in pregnant rats

Figures & data

Table 1. Serum and urine biochemical parameters of control group, EDS-treated groups (EDS) and EDS treated animals supplemented with BJ0021 (EDS + BJ0021) at the end of experimental period.

	Experimental groups
	Control (n = 10)	Endo (n = 10)	Endo + BG021 (n = 10)
Parameters	Mean ± SEM	Mean ± SEM	Mean ± SEM
Serum
Cholesterol (g/L)	1.72 ± 0.77	2.78* ± 0.50	1.83^b ± 0,62
ASAT (U/L)	103.50 ± 0.50	1500* ± 5,20	117^b ± 05.02
ALAT (U/L)	73.70 ± 06.30	179.4* ± 7.4	57.8^a ± 5.7
ALP(U/L)	183.3 ± 16.9	190.3 ± 29.00	139.8 ± 18.4
GGT (U/mg protein)	0.03 ± 0.002	0.11* ± 0.04	0.08^a ± 0.01
Urea (g/L)	0.38 ± 0.003	0.42* ± 0.03	0.37^a ± 0.02
Creatinine (mg/L)	9,15 ± 0,29	11.27* ± 0.39	10.16 ± 0.43
Urine
Urea (mg/24 h)	1.08 ± 0.03	0.64** ± 0.05	1.24^a ± 0.13
Creatinine (mg/kg/24 h)	0.392 ± 0.10	0.36 ± 0.09	0.403 ± 0.14

Notes: Statistically significant differences from control: *p < 0.05, **p < 0.01; from EDS + BJ0021: ^ap < 0.05, ^bp < 0.01; Values are given as mean ± SEM, n = 10 number of animals.

Figure 1. CAT, SOD activities and MDA level in liver and kidney tissues of control, EDS and EDS + BJ0021 groups at the end of treatment. Notes: Statistically significant differences from control: *p < 0.05, **p < 0.01; from EDS + BJ0021: ^ap < 0.05, ^bp < 0.01. Values are given as mean ± SEM, n = 10 number of animals.

Figure 2. TUNEL staining cells cross section counterstained with hematoxylin. Notes: Arrows point to brown stained apoptotic cells. (A) Kidney section. Magnification 40 × . (B) Liver section. Magnification 100×. (a) Negative control, (b) Control, (c) EDS treatment, (d) EDS + BJ0021 treatment. (C) Number of apoptotic nucleus obtained from liver and kidney by TUNEL method in contol, EDS and EDS + BJ0021 groups. Notes: Statistically significant differences from control: *p < 0.05, ***p < 0.001; from EDS + BJ0021: ^ap < 0.05, ^bp < 0.01. Values are given as mean ± SEM, Number of animals n = 10.