Figures & data
Table I. Antimicrobial spectrum of CFC filtrate and EPC of Lact. rhamnosus 231.
Table II. Effects of proteinase K and temperature on the activity of EPC of Lact. rhamnosus 231.
Table III. Effect of pH on the activity of EPC of Lact. rhamnosus 231 against gram-positive and gram-negative bacteria.
Figure 1. Survival of Lact. rhamnosus 231 cells exposed in the presence or absence of bile salt (2 and 4%), NaCl (4 and 8%) and at pH (2.5 and 3.5) for 4 h at 37°C.
Figure 2(a). Growth profile of Lactobacillus rhamnosus 231 in MRS medium at 37°C.
Figure 2(b). Antimicrobial activity of CFC filtrate (A& C) and EPC (B&D) against different test organisms indicated as a legend of each graph. Antimicrobial activity was measured as zone of inhibition (mm) around punched hole (7 mm).
Figure 3. Tricine-SDS-PAGE gels of partially purified extracellular protein(s) from Lactobacillus rhamnosus 231 cultures growing on MRS medium. (Citation1) Lane (A) Low molecular weight standards (Bio-Rad, Hercules, CA), EPC stained with (lane B) “silver” and (lane C) “PAS” stain. (Citation2) Inhibition of growth of Bacillus cereus (lane B) and Ps. aeruginosa 105 (lane C) by low molecular weight glycoprotein, which corresponds to the band observed in lane A.
Table IV. Purification table of extracellular antimicrobial proteins produced by Lact. rhamnosus 231.
Figure 4. Superose 12HR 10/30 chromatogram of the EPC from 18 h old cultures of L. rhamnosus 231 growing on MRS medium. Inset shows Tricine-SDS-PAGE of partially purified glycopeptides eluted during SEC chromatography. Lane 1. Low molecular weight standards (Bio-Rad, Hercules, CA), lane 2. EPC after ammonium sulfate precipitation, lane 3 to 7, five pooled fractions 4–6, 7–8, 9–10, 11–13 and 14–16, respectively of EPC eluted during size exclusion chromatography.
