Biochemical and antimicrobial profile of Enterococcus faecium and E. faecalis isolated from traditional dairy products and infant intestinal microbiota

Figures & data

Table I. Origin of E. faecium and E. faecalis strains tested in the study.

Origin of samples	No. of samples	S. faecium and S. faecalis strains isolated
Unpasteurized ewe milk	20	SFC2, SFL2, SFL3
Unpasteurized goat milk	10	SFC1
Unpasteurized cow milk	10
Feta cheese	20	SFL1
Goat cheese	20	SFC4, SFC5, SFL4, SFL5
Rennet (pitia)	20	SFC3, SFC6, SFC7, SFC8, SFC9, SFC10, SFC11, SFC12, SFC13, SFL6, SFL7, SFL8, SFL9, SFL10, SFL11
Infant faeces	20	CFC14
Total	120	25

SFC denotes an E. faecium strain; SFL denotes an E. faecalis strain.

Table II. Antibiotic susceptibility^* profiles of E. faecium and E. faecalis isolates from traditional dairy products and infant faeces.

No. of Enterococcus strains isolated	Vancomycin	Gentamicin HLAR	Ampicillin	Erythromycin	Tetracycline	Teicoplanin	Ciprofloxacin
SFC1	R	S	R	S	R	R	S
	R	S	R	R	S	I	R
SFC3	S	S	S	S	S	I	R
SFC4	R	I	R	I	R	R	R
SFC5	R	R	S	R	S	R	I
SFC6	R	I	S	S	S	R	I
SFC7	I	S	S	R	S	R	S
SFC8	I	I	S	S	S	S	R
SFC9	R	R	R	I	S	I	S
SFC10	I	I	I	S	S	S	R
SFC11	R	I	R	R	R	I	I
SFC12	R	S	S	S	S	R	S
SFC13	I	S	S	R	S	R	S
SFC14	R	R	R	R	R	R	R
SFC15 reference strain	R	R	R	I	S	S	S
SFL1	S	S	I	S	S	S	I
SFL2	I	I	I	S	S	S	S
SFL3	R	R	R	R	S	R	S
SFL4	R	S	R	R	R	R	I
SFL5	I	S	R	R	S	I	I
SFL 6	S	S	S	S	I	I	R
SFL 7	I	S	S	R	S	S	S
SFL 8	R	I	S	R	S	R	R
SFL 9	S	S	S	S	S	S	S
SFL10	I	R	I	S	R	S	S
SFL11	R	S	S	S	I	I	S
SFL12 reference strain	R	I	R	S	S	S	R

SFC denotes a strain of E. faecium; SFL denotes a strain of E. faecalis.

^*According to the National Committee for Clinical Laboratory Standards, 2006: R, resistant; I, intermediate; S, susceptible.

Table III. Antimicrobial activities (mm of inhibition zone) of selected Enterococcus strains toward pathogenic indicator bacteria at 37°C or 45°C for 24 h cultures/supernatant liquid/l actic acid.

	Inhibition zone (mm)
Enterococcus stain	S. typhi	S. typhimurium	S. ancona	S. enteritidis
SFC1	11/6/8	15/7/9	10/5/6	12/7/9
	9/7/8	12/7/8	9/5/6	10/7/9
SFC2	12/7/9	16/7/10	10/6/6	9/5/10
	10/7/8	14/6/9	10/6/6	9/5/10
SFC3	16/7/9	19/7/10	8/5/5	11/5/10
	12/8/8	15/7/10	8/5/5	10/5/10
SFC4	12/7/9	14/7/9	10/6/5	9/5/9
	10/7/8	13/6/10	9/5/5	8/5/8
SFC5	13/8/10	13/5/8	5/5/5	18/8/10
	10/6/7	12/5/8	5/5/5	17/6/9
SFC6	19/8/9	19/5/9	12/7/6	20/5/10
	13/7/10	14/5/9	10/6/6	16/5/10
SFC7	22/7/8	20/7/10	13/6/7	13/5/5
	12/5/9	17/7/10	12/6/6	10/5/5
SFC8	25/8/9	22/5/11	11/5/7	12/5/10
	15/7/9	18/5/9	11/5/6	10/5/9
SFC9	15/7/8	15/7/12	15/6/7	11/6/10
	12/7/7	14/7/9	14/5/6	11/5/9
SFC10	23/5/9	23/5/7	5/5/5	15/5/9
	15/5/10	24/5/7	5/5/5	14/5/8
SFC11	29/8/12	17/8/9	7/5/5	12/5/10
	23/10/11	16/8/9	7/5/5	10/5/10
SFC12	18/5/9	18/7/10	8/5/5	14/7/10
	15/5/9	17/7/10	8/5/5	13/7/10
SFC13	28/8/9	20/5/8	10/6/5	12/5/9
	21/5/9	17/5/8	9/5/5	10/5/8
SFC14	13/5/7	12/6/9	13/6/5	9/6/8
	7/5/7	11/5/8	13/5/5	9/6/7
SFC15 reference strain	14/5/8	12/7/10	7/5/5	10/5/8
	12/10/11	11/7/9	6/5/5	10/5/8
SFL1	15/10/13	14/10/12	13/9/5	12/5/9
	19/12/16	16/10/14	13/9/10	16/9/14
SFL2	19/12/16	15/10/14	12/9/10	15/9/12
	20/15/18	20/11/17	14/9/12	17/9/15
SFL3	14/9/13	15/11/12	9/5/5	13/5/10
	15/11/13	14/9/11	9/5/5	13/5/12
SFL4	15/5/13	13/2/10	9/5/5	12/5/9
	16/12 /14	15/9/11	11/5/8	15/5/11
SFL5	14/5/12	13/5/11	5/5/5	11/5/5
	15/5/12	15/9/11	5/5/5	12/5/9
SFL6	17/5/12	15/5/11	12/5/5	11/5/6
	16/11/12	14/5/11	11/5/5	12/5/10
SFL7	18/11/14	15/11/10	15/5/5	15/8/10
	18/11/14	16/10/11	11/8/8	15/5/11
SFL8	18/5/12	14/9/12	12/5/5	15/5/10
	18/12/16	15/5/12	5/5/11	15/9/11
SFL9	15/5/11	15/9/10	11/5/5	12/5/9
	17/5/12	15/5/12	11/5/5	15/5/10
SFL10	17/5/13	15/5/10	12/5/5	12/5/9
	16/5/12	15/5/12	5/5/9	15/5/10
SFL11	15/5/11	15/5/11	13/5/8	12/5/9
	14/5/10	13/5/10	11/5/6	15/5/9
SFL12 reference strain	13/5/11	12/5/10	5/5/5	5/5/5
	14/5/10	11/5/10	5/5/5	5/5/5

For each strain, incubation for 24 h at 37°C is reported on the first line, and incubation for 24 h at 45°C is reported on the second line. SFC denotes a strain of E. faecium; SFL denotes a strain of E. faecalis.

Figure 1. Inhibition zones (mm) of cultures, supernatant liquid and lactic acid of E. faecium and E. faecalis against four pathogenic bacteria (S. ancona, S. enteritidis, S. typhimurium, S. typhi). **Analysis of variance test results (p < 0.05).