Hydrogen sulfide changes adhesive properties of fibrinogen and collagen in vitro

Figures & data

Table I.  Platelet adhesion of resting and thrombin-stimulated platelets to microplate wells coated with native adhesive proteins (fibrinogen and collagen) and NaHS-treated fibrinogen or collagen (5 min, 15 min, and 30 min, 37°C).

Concentration of NaHS [mM]	Adhesion of resting platelets [%]	Adhesion of thrombin–activated platelets [%]
Incubation time–5 minutes
Native collagen [0 mM NaHS]	100	100
Collagen + 0.00001  mM NaHS	96.2 ± 7.4	95.7 ± 7.8
Collagen + 0.0001 mM NaHS	92.3 ± 7.6	92.1 ± 5.9
Collagen + 0.001 mM NaHS	87.3 ± 8.8	87.4 ± 6.9
Collagen + 0.01 mM NaHS	81.5 ± 7.6	83.3 ± 10.1
Collagen + 0.1 mM NaHS	79.3 ± 8.1	79.4 ± 7.7
Collagen + 1 mM NaHS	75.4 ± 7.0	70.3 ± 6.5
Collagen + 5 mM NaHS	71.4 ± 9.0	65.2 ± 5.9
Collagen + 10  mM NaHS	66.6 ± 4.9	60.4 ± 4.9
Native fibrinogen [0 mM NaHS]	100	100
Fibrinogen + 0.00001 mM NaHS	93 ± 5.4	87.5 ± 10.8
Fibrinogen + 0.0001 mM NaHS	86.9 ± 9.9	82.0 ± 8.6
Fibrinogen + 0.001 mM NaHS	83.7 ± 12.1	79.8 ± 13.7
Fibrinogen + 0.01 mM NaHS	80.7 ± 10.6	74.2 ± 12.7
Fibrinogen + 0.1 mM NaHS	76.3 ± 10.5	69.7 ± 13.6
Fibrinogen + 1 mM NaHS	74.9 ± 8.9	63.2 ± 12.2
Fibrinogen + 5 mM NaHS	70.4 ± 10.2	51.8 ± 8.2
Fibrinogen + 10 mM NaHS	61.5 ± 5.6	49.0 ± 8.9
Incubation time–15 minutes
Native collagen [0 mM NaHS]	100	100
Collagen + 0.01  mM	74.7 ± 8.2	75.9 ± 5.9
Collagen + 10 mM	53.2 ± 5.5	54.3 ± 4.1
Native fibrinogen [0 mM NaHS]	100	100
Fibrinogen + 0.01 mM	70.5 ± 4.9	67.9 ± 7.2
Fibronogen + 10 mM	49.4 ± 7.8	40.4 ± 6.6
Incubation time–30 minutes
Native collagen [0 mM NaHS]	100	100
Collagen + 0.01  mM	70.2 ± 5.9	69.9 ± 7.3
Collagen + 10 mM	44.3 ± 7.1	47.8 ± 4.5
Native fibrinogen [0 mM NaHS]	100	100
Fibrinogen + 0.01 mM	63.7 ± 4.9	60.2 ± 6.6
Fibrinogen + 10 mM	41.3 ± 6.6	38.5 ± 7.4

Notes: Results represent the means of eight values. The effect of nine different concentrations of NaHS (0.00001, 0.0001, 0.001, 0.01 0.1, 1, 5, and 10 mM) vs. control was statistically significant according ANOVA test and Post Hoc test–(for collagen–p < 0.01 (for resting platelets), p < 0.001 (for stimulated platelets); for fibrinogen–p < 0.02 (for resting platelets), p < 0.0001 (for stimulated platelets)).