Figures & data
Figure 1. The effect of TFP on the Laurdan generalized polarization dependence on the excitation wavelength in DOPC:SM:Chol (1:1:1) liposomes. Symbols represent: – pure lipid,
– lipid + 50 μM of TFP, Δ – lipid + 100 μM of TFP,
– lipid + 200 μM of TFP. Measurements were performed at 25°C (A) and 50°C (B).
Figure 2. Representative confocal images (hemispherical projections) of GUVs formed from equimolar DOPC:SM:Chol mixture (A), and GUVs modified with 200 μM of TFP (B-D). GUVs were labelled with DiIC12(3) (probe concentration 0.1 mol %). Scale bar is 10 μm. Images were recorded at room temperature.
Figure 3. The number of domains observed in GUVs formed from DOPC:SM:Chol (1:1:1) under control conditions, modified with 100 μM of TFP, 200 μM of TFP, 200 μM of TDZ, and 200 μM of CPZ (from left to right, respectively). Median values (squares) and 25–75% values (boxes) are shown. An asterisk marks the results significantly different from the control (p < 0.05).
Figure 4. Domain's area in GUVs formed from DOPC:SM:Chol (1:1:1) under control conditions, modified with 100 μM of TFP, 200 μM of TFP, 200 μM of TDZ, and 200 μM of CPZ (from left to right, respectively). Median values (squares) and 25–75% values (boxes) are shown. An asterisk marks the results significantly different from the control (p < 0.05).
Figure 5. The total length of domains' border recorded in GUVs formed from DOPC:SM:Chol (1:1:1) under control conditions, modified with 100 μM of TFP, 200 μM of TFP, 200 μM of TDZ, and 200 μM of CPZ (from left to right, respectively). Median values (squares) and 25–75% values (boxes) are shown. An asterisk marks the results significantly different from the control (p < 0.05).
