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Stress
The International Journal on the Biology of Stress
Volume 13, 2010 - Issue 3
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Research Article

Cytokine and chemokine gene expression profiles in heterophils from chickens treated with corticosterone

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Pages 185-194 | Received 16 Mar 2009, Accepted 25 Jun 2009, Published online: 03 Dec 2009

Figures & data

Table I.  Real-time quantitative RT-PCR primers and probes.

Figure 1. Fold-changes in cytokine mRNA levels in peripheral heterophils from corticosterone-treated chickens at 3 h, 24 h and 1 week after initial exposure when compared to controls (untreated and ethanol [vehicle] treated birds). Error bars show SEM from triplicate samples (n = 16 chickens per group) from two separate qRT-PCR experiments; p < 0.01.

Figure 1.  Fold-changes in cytokine mRNA levels in peripheral heterophils from corticosterone-treated chickens at 3 h, 24 h and 1 week after initial exposure when compared to controls (untreated and ethanol [vehicle] treated birds). Error bars show SEM from triplicate samples (n = 16 chickens per group) from two separate qRT-PCR experiments; p < 0.01.

Figure 2. Fold-change in chemokine mRNA levels in peripheral heterophils from corticosterone-treated chickens at 3 h, 24 h and 1 week after initial exposure when compared to controls (untreated and ethanol [vehicle] treated birds). Error bars show SEM from triplicate samples (n = 16 chickens per group) from two separate qRT-PCR experiments; p < 0.01.

Figure 2.  Fold-change in chemokine mRNA levels in peripheral heterophils from corticosterone-treated chickens at 3 h, 24 h and 1 week after initial exposure when compared to controls (untreated and ethanol [vehicle] treated birds). Error bars show SEM from triplicate samples (n = 16 chickens per group) from two separate qRT-PCR experiments; p < 0.01.

Table II.  Effects of administration of corticosterone in drinking water on plasma corticosterone concentrations* and heterophil counts at 0 h, 3 h, 24 h and 1 week post-treatment.

Table III.  Correlation of plasma corticosterone concentrations and total circulating heterophils with heterophil cytokine and chemokine mRNA expression levels.

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