Acute restraint stress increases the frequency of vinblastine-induced micronuclei in mouse bone marrow cells

Figures & data

Figure 1.  Mitotic blocked bone marrow cells versus interphase cells.

Figure 2.  Micronucleated polychromatic erythrocyte (MNPCE).

Table I.  Effect of different doses of vinblastine on MNPCE-induction.

Dose of vinblastine (mg/kg BW)	Hours post-vinblastine treatment	MNPCEs in 100 PCEs (means+SEM)	MI in 100 nucleated cells (means+SEM)
0	–	1.12 ± 0.47^*	0.00^*
1	6	2.28 ± 0.64^*,^*^*	0.00^*
2	6	2.44 ± 0.72^*^*	0.39 ± 0.11^*
	18	3.18 ± 1.08^*^*	–
	24	6.24 ± 1.11^*^*^*	–
	30	5.87 ± 1.10^*^*^*	–
3	6	4.31 ± 1.17^*^*^*	4.98 ± 1.78^*^*
5	6	3.01 ± 0.65^*^*,^*^*^*	5.14 ± 2.79^*^*

Cells from both femurs of four mice were analyzed for each treatment. MNPCEs, micronucleated polychromatic erythrocytes; PCEs, polychromatic erythrocytes; MI, mitotic index. ^*,^**,^***Significantly different (p < 0.01).

Table II.  Effect of restraint stress on MNs induction in bone marrow cells.

	MNPCEs in 100 PCEs (means+SEM)	PCE/PCE+NCE (means+SEM)
Control	1.73 ± 0.23^*	49.98 ± 0.75
Stress	2.93 ± 1.79^*,^*^*	50.23 ± 1.12
Vinblastine treatment	4.07 ± 0.61^*^*	51.12 ± 0.95
Stress+vinblastine treatment	14.26 ± 3.31^*^*^*	48.56 ± 1.27

Cells from both femurs of four mice were analyzed for each treatment. MNPCEs, micronucleated polychromatic erythrocytes; PCEs, polychromatic erythrocytes; NCE, normochromatic erythrocyte. ^*,^**,^***Significantly different (p < 0.01).