Amphiphilic polyaspartamide copolymer-based micelles for rivastigmine delivery to neuronal cells

Figures & data

Figure 1.  Chemical structure of PHEA-EDA-Sq₁₇-PS₈₀ copolymer (x + y + z + n = 2).

Figure 2.  I₃₃₈/I₃₃₂ intensity ratio calculated from pyrene excitation spectra and I₁/I₃ intensity ratio calculated from pyrene emission spectra in water, as a function of the logarithm of copolymer concentration.

Figure 3.  Contour plot of the NOESY experiment on PHEA-EDA-Sq₁₇-PS₈₀ copolymer micelles recorded with an experimental mixing time of 350 ms.

Table 1.  Mean diameter, polydispersity index (PDI) and ζ potential of unloaded and Riv-loaded PHEA-EDA-Sq₁₇-PS₈₀ micelles.

Sample	Mean diameter (nm)	PDI	ζ Potential (mV ± SD)
Unloaded micelles	28.7	0.26	−18.2 ± 6.35
Riv-loaded micelles	34.1	0.35	−18.1 ± 5.5

Figure 4.  Cell viability of Neuro2a cells incubated for 6 h (a), 24 h (b) and 48 h (c) with free Riv (drug), Riv-loaded micelles (drug-mic) and unloaded micelles (empty mic). Each bar represents the mean of three sample ± SD.

Figure 5.  Neuro2a uptake of free Riv (drug) and Riv-loaded micelles (drug-loaded mic) after 6 h incubation.

Figure 6.  Stability profile of Riv-loaded into PHEA-EDA-Sq₁₇-PS₈₀ micelles (drug-loaded mic) after incubation in human plasma for 6, 24 and 48 h, in comparison with free rivastigmine (drug).