Development of gentamicin-gold nanospheres for antimicrobial drug delivery to Staphylococcal infected foci

Figures & data

Figure 1.  Chemical structure of gentamicin sulfate.

Figure 2.  Gold nanospheres before (A) and after (B) conjugation reaction with gentamicin.

Figure 3.  UV-Vis spectra of gentamicin, gold nanospheres and their respective conjugates. A shift in the maximum is representative for the production of gentamicin-gold nanospheres complex.

Figure 4.  Scheme of conjugation of GNPs with gentamicin.

Table 1.  Results of atomic absorption analysis.

Sample	Concentration Au (mg/l)
Gold nanospheres	4.96
Gold nanospheres attached to gentamicin	0.14

Figure 5.  HPLC chromatogram of unbounded gentamicin in the supernatant solution.

Table 2.  The MIC and MBC of gentamicin and a gentamicin-gold nanospheres complex against S. aureus.

Sample	MIC (mg/ml)	MBC (mg/ml)
Gentamicin	0.1875	0.375
Gentamicin-GNPs	0.09375	0.1875

Figure 6.  Biodistribution data at 10, 20, 30 and 60 min after injection of gentamicin-gold nanospheres complex in infected mouse model.