Figures & data
Table 1. Characterization of coumarin-6 liposomes with different PC composition (n = 3).
Figure 1. The flow cytometric measurement of coumarin-6 uptake from NGR-SL-C-HS and SL-C-HS by HT1080 (CD13+) cells at the time of 1 h. Cells were incubated with NGR-SL-C-HS and SL-C-HS at the final coumarin-6 concentration of 100 ng/mL, and the time point of 1 h, the cells were trypsinized, washed and analyzed using flow cytometry. 1 – Control, 2 – SL-C-HS, 3 – NGR+NGR-SL-C-HS, 4 – NGR-SL-C-HS.
Figure 2. The flow cytometric measurement of coumarin-6 uptake from NGR-SL-C-HS, NGR-SL-C-S, NGR-SL-C-H and NGR-SL-C-D by HT1080 (CD13+) cells at the time of 1 h. Cells were incubated with liposomes at the final coumarin-6 concentration of 100 ng/mL. And the time point of 1 h, the cells were trypsinized, washed and analyzed using flow cytometry. 1 – Control, 2 – NGR-SL-C-D, 3 – NGR -SL-C-H, 4 – NGR-SL-C-HS, 5 – NGR-SL-C-S.
Figure 3. The confocal microscopy images of HT1080 (CD13+) and HCAEC (CD13−) incubated with different coumarin-6 liposomes for 1 h at 37 °C. Green represents fluorescence of coumarin-6, blue represents fluorescence of Hoechst 33258. (For interpretation of the reference to color in this figure legend, the reader is referred to the web version of this article.)
Table 2. Characterization of brucine-loaded liposomes with different lipid composition (n = 3).
Figure 4. The release of brucine from NGR-SL-B with different lipid compositions and SL-B-HS incubated with fresh rat plasma at 37 °C (n = 3).
Figure 5. Cytotoxicity of brucine-loaded liposomes with different lipid composition and brucine solution. After the addition of brucine formulations (NGR-SL-B-HS, SL-B-HS, NGR-SL-B-S, NGR-SL-B-H, NGR-SL-B-D or brucine solution) to cells, the mixture was incubated for 48 h and the viability of HT1080 cells was measured by MTT assay.
Figure 6. The ex vivo optical images of tumors and organs of HT1080 in tumor-bearing mice sacrificed at 20 h after NGR-SL-DiR with different lipid composition administration.
