Ovarian cancer targeted hyaluronic acid-based nanoparticle system for paclitaxel delivery to overcome drug resistance

Figures & data

Figure 1. Scheme diagram to illustrate the preparation of HA-PTX-NLCs.

Table 1. Characterization of different vectors.

Samples	Blank NLCs	PTX-NLCs	HA-PTX-NLCs
Particle size (nm)	93.6 ± 3.1	96.1 ± 3.4	114.3 ± 4.6
Size distribution	0.10 ± 0.02	0.13 ± 0.02	0.16 ± 0.03
Zeta potential (mV)	＋17.9 ± 2.3	＋29.6 ± 3.4	＋11.5 ± 1.7
EE (%)	N/A	83.1 ± 3.2	81.9 ± 2.8
LC (%)	N/A	5.6 ± 0.6	3.2 ± 0.5

Table 2. Optimization of HA-PTX-NLCs.

HA-DSPE to PTX-NLCs ratio (w/w)	Zeta potential (mV)	Particle size (nm)	EE (%)
1:3	＋24.3 ± 4.1	99.7 ± 3.4	82.3 ± 2.6
1:2	＋18.6 ± 2.8	105.3 ± 3.7	81.7 ± 2.7
1:1	＋11.7 ± 1.9	113.4 ± 4.6	82.4 ± 2.4
2:1	＋10.1 ± 2.6	237.6 ± 45.2	61.2 ± 6.4
3:1	＋9.9 ± 2.2	339.9 ± 64.7	38.3 ± 8.2

Figure 2. PTX release from HA-PTX-NLCs, PTX-NLCs, and PTX solution in PBS 7.4.

Figure 3. PTX release from HA-PTX-NLCs, PTX-NLCs, and PTX solution in PBS 5.0.

Table 3. Stability of HA-PTX-NLCs in 50% FBS.

Samples	Particle size (nm)	Size distribution	Zeta potential (mV)
HA-PTX-NLCs	118.7 ± 5.9	0.19 ± 0.05	− 17.6 ± 2.4
PTX-NLCs	101.4 ± 4.2	0.15 ± 0.03	−8.6 ± 0.9

Figure 4. Cell viability of HA-PTX-NLCs, PTX-NLCs, and PTX solution in SKOV3 cells.

Figure 5. Cell viability of HA-PTX-NLCs, PTX-NLCs and PTX solution in SKOV3/PTX cells.

Table 4. IC₅₀ of HA-PTX-NLCs, PTX-NLCs, PTX solution evaluated in SKOV3 cells and SKOV3/PTX cells.

Samples	HA-PTX-NLCs	PTX-NLCs	Free PTX
SKOV3 cells (µM)	0.011 ± 0.003	0.024 ± 0.006	0.216 ± 0.031
SKOV3/PTX  cells (µM)	0.019 ± 0.004	0.045 ± 0.008	23.156 ± 2.351

Figure 6. The tumor growth inhibition effect of different groups.

Table 5. Tumor inhibition rates of samples in ovarian cancer bearing mice.

Samples	HA-PTX-NLCs	PTX-NLCs	Free PTX
TIR (%)	85.0 ± 4.1	72.6 ± 3.3	25.1 ± 1.9

Figure 7. Body weight changes of different groups.

Figure 8. Mean tissue concentrations of PTX after injection of PTX solution.

Figure 9. Mean tissue concentrations of PTX after injection of HA-PTX-NLCs.