Development of Serratiopeptidase and Metronidazole Based Alginate Microspheres for Wound Healing

Figures & data

Table 1. Composition and properties of the alginate microspheres

Concentration of polymer	Product Code	Type of amount	Percentage encapsulation efficiency (%)	% Yield	Mean size diameter (μm)	% Degree of aggregation
Metronidazole
Sodium alginate 2% w/w	MA1	40% CaCl2	62.7	72.72	104	17
	MA2	20% CaCl2	27.8	62.27	376	37
	MA3	30% CaCl2	74.73	76.13	72	4
Sodium alginate 2.5% w/w	MB1	40% CaCl2	65.6	76.85	102	12
	MB2	20% CaCl2	30.93	70.55	373	35
	MB3	30% CaCl2	81.0	82.59	70	3
Sodium alginate 3% w/w	MC1	40% CaCl2	61.87	71.09	104	17
	MC2	20% CaCl2	28.7	61.00	379	41
	MC3	30% CaCl2	73.8	74.37	73	7
Serratiopeptidase
Sodium alginate 2% w/w	SA1	40% CaCl2	60.67	71.36	107	16
	SA2	20% CaCl2	22.82	58.18	381	38
	SA3	30% CaCl2	69.48	74.09	79	5
Sodium alginate 2.5% w/w	SB1	40% CaCl2	63.2	74.26	103	11
	SB2	20% CaCl2	25.23	69.25	375	40
	SB3	30% CaCl2	73.6	81.48	74	4
Sodium alginate 3% w/w	SC1	40% CaCl2	57.9	70.06	110	19
	SC2	20% CaCl	23.8	59.43	383	47
	SC3	30% CaCl2	68.3	73.28	81	10

Figure 1. Cumulative % release of metronidazole (MB) and serratiopeptidase (SB) from alginate microspheres.

Figure 2. Photographs of wounds 5, 10, 15 and 20 days after surgery illustratating the changes in capillary permeability and wound contraction. (A) control; (B) reference; (C) serratiopeptidase; (D) serratiopeptidase and metronidazole.

Figure 3. Photomicrographs of histologic sections of 5, 10, 15 and 20 days old wound. (A) control; (B) reference; (C) serratiopeptidase; (D) serratiopeptidase and metronidazole.

Table 2. Distance (cm) between the wound edge and the line two centimeter apart from the wound at different intervals

	Observation days
Choice	5	10	15	20
Control	2	2.2	2.4	2.4
Marketed	2	2.2	2.4	2.5
Serratiopeptidase	2	2.2	2.4	2.5
Serratiopeptidase and metronidazole	2	2.2	2.7	2.9

Table 3. Collagen (mg) content per 100 mg of the wound bed

	Observation days
Choice	5	10	15	20
Control	9.1	18.907	44.66	41.09
Marketed	14.707	29.421	63	53.907
Serratiopeptidase	14.609	29.246	59.017	53.284
Serratiopeptidase and metronidazole	15.435	35.735	66.157	58.884