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Research Article

Properties of immobilized glucose oxidase and enhancement of enzyme activity

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Pages 264-268 | Received 16 Aug 2012, Accepted 10 Sep 2012, Published online: 30 Oct 2012

Figures & data

Table I. Enzyme activity values of the free and immobilized GOD.

Figure 1. The activity of free and immobilized glucose oxidase at different pH values.

Free enzyme
immobilized enzyme.

Figure 1. The activity of free and immobilized glucose oxidase at different pH values. Display full sizeFree enzyme Display full sizeimmobilized enzyme.

Figure 2. The activity of free and immobilized glucose oxidase at different temperature values.

Free enzyme
immobilized enzyme.

Figure 2. The activity of free and immobilized glucose oxidase at different temperature values. Display full sizeFree enzyme Display full sizeimmobilized enzyme.

Figure 3. Effect of substrate concentration on the initial velocity of glucose oxidation.

Immobilized enzyme
free enzyme.

Figure 3. Effect of substrate concentration on the initial velocity of glucose oxidation. Display full sizeImmobilized enzyme Display full sizefree enzyme.

Figure 4. Lineweaver-Burk plot for the free and immobilized enzyme.

Immobilized enzyme
free enzyme.

Figure 4. Lineweaver-Burk plot for the free and immobilized enzyme. Display full sizeImmobilized enzyme Display full sizefree enzyme.

Table II. Kinetic parameters of the free and immobilized GOD.

Figure 5. The effect of silicone oil concentration on immobilized glucose oxidase.

Figure 5. The effect of silicone oil concentration on immobilized glucose oxidase.

Figure 6. The effect of soybean oil concentration on immobilized glucose oxidase.

Figure 6. The effect of soybean oil concentration on immobilized glucose oxidase.

Figure 7. Repetitive use of immobilized glucose oxidase.

Figure 7. Repetitive use of immobilized glucose oxidase.

Figure 8. Storage stability of immobilized glucose oxidase at 4°C.

Figure 8. Storage stability of immobilized glucose oxidase at 4°C.

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