Effects of pearl powder extract and its fractions on fibroblast function relevant to wound repair

Figures & data

Table 1.  Yield and protein content of the extract and fractions.

	Weight (mg/100g pearl powder)	Protein content (%)
WSM	92.0 ± 4.23	12.16 ± 1.86
MR14	88.2 ± 5.87	18.90 ± 1.94
MR3–14	67.8 ± 3.59	15.16 ± 1.12
MR3	—	2.92 ± 0.48

Values are expressed as mean ± SEM (n = 5).

Figure 1.  Morphological appearance of mouse oral fibroblasts: (A) fibroblasts in primary culture; (B) fibroblasts subcultured to the second generation; (C) fibroblasts subcultured to the third generation.

Figure 2.  Effects of WSM, MR14, MR3–14, and MR3 on the growth of fibroblasts (μg/mL): (A) after 48 h incubation, (B) after 72 h incubation. Values shown represent the mean ± SEM (n = 5). *p < 0.05, **p < 0.01.

Figure 3.  Effects of WSM, MR14, MR3–14, and MR3 on growth curves of fibroblasts. Data represent the mean ± SEM (n = 5). *p < 0.05, **p < 0.01.

Figure 4.  Effects of WSM, MR14, MR3–14, and MR3 on collagen production. Results are expressed as the mean ± SEM (n = 5) *p < 0.05, **p < 0.01.

Figure 5.  Effects of WSM, MR14, MR3–14, and MR3 on MMP-2, -9 activities. (A) Results of gelatin zymography. Lane 1: WSM; lane 2: MR14; lane 3: MR3–14; lane 4: MR3; lane 5: 1% FCS control; lane 6: 10% FCS control. (B) Activities of MMP-2, -9 expressed as multiplication product of zone area and average gray value. Data represent the mean ± SEM (n = 5). *p < 0.05, **p < 0.01.

Figure 6.  Effects of WSM, MR14, MR3–14, and MR3 on the production of TIMP-1, Data are expressed as the mean ± SEM (ng/mL). *p < 0.05, **p < 0.01.