Effect of bFGF on invasion of ovarian cancer cells through the regulation of Ets-1 and urokinase-type plasminogen activator

Figures & data

Figure 1.  RT-PCR analysis of uPA and Ets-1 genes. After SKOV₃ cells were cultured in basal medium and different concentrations of bFGF (0.1, 1, 10, 100 ng/mL) for 24 h, a significant change of expression of uPA and Ets-1 mRNA of SKOV₃ cells was observed. The increase of both genes presented a dose-dependent response. β-actin was functioning here as a housekeeping gene.

Figure 2.  Effect of bFGF on secretion of uPA protein. Each bar represents mean ± SEM. After treatment with different concentrations of bFGF (0.1, 1, 10, 100 ng/mL), supernantant was obtained from cultures of SKOV₃ cells, and tested by ELISA. The expression of uPA protein increased due to stimulation with bFGF in a dose-dependent manner.

Table 1.  Effect of bFGF on cell invasion of SKOV₃ cells and the effect of antisense oligonucleotides on that procedure.

Treatment	Migrated cells	Fold increase (vs. control)
Basal medium (control)	1.9 ± 0.7
10 ng/mL bFGF	4.4 ± 1.0	2.3
10 ng/mL bFGF + sense Ets-1 oligonucleotides	4.5 ± 1.0	2.4
10 ng/mL bFGF + antisense Ets-1 oligonucleotides	2.9 ± 0.9	1.5