Antiproliferative effect of extracts from Aristolochia baetica and Origanum compactum on human breast cancer cell line MCF-7

Figures & data

Table 1.  Ethnobotanical data and antiproliferative effect of extracts from the studied plants.

Plant	Part used	Local name	Site of collection	Local use	Extract	IC50 (μg/ml)
						Time of treatment (h)
						24	48	72
Aristolochiaceae A. baetica	Root, leaf	Zraouand	Chefchaoun	Anti-inflammation, antiseptic, antirheumatic, abortive, circulatory stimulant, cutaneous neoplasm, internal tumors*	Hexane	352.96 ± 20	251.74 ± 18	168.5 ± 14
					Chloroform	441.6 ± 23	216.06 ± 15	146.65 ± 13
					Ethyl acetate	>1000	608.53 ± 29	435.64 ± 21
					Methanol	Inactive	Inactive	Inactive
LamiaceaeO. compactum	Aerial part	Zaâtar	Chichawa	Vermifuge, aphrodisiac, condiment, antispasmodic, antidiarrheal, antiacid, sedative, internal tumors*	Hexane	**	**	**
					Chloroform	**	**	**
					Ethyl acetate	281.11 ± 15	279.51 ± 16	275.05 ± 14
					Methanol	575.57 ± 20	382.12 ± 17	374.01 ± 16
Positive control						< 5	< 5	< 5

IC₅₀ (inhibitory concentration 50%) and SD (standard deviation for 95% confidence): was determined from the linear regression curve of cell proliferation inhibition on MCF-7 cells versus plant extracts. The range of concentrations assayed was 1 to 1000 μg/mL. Paclitaxel was used as a positive control and exhibited an IC₅₀ value of less than 5 μg/mL for the three treatment times.

*Oral interview with expert herbalists.

**Non exploitable.

Figure 1.  Antiproliferative effect of Aristolochia baetica L extracts on MCF-7 cells. Cells were treated with various concentrations for 24 (—), 48 (-·-), and 72 h (…). (A) MCF-7 cells are treated by hexane extract. (B) MCF-7 cells are treated by chloroform extract. (C) MCF-7 cells are treated by ethyl acetate extract. Results are presented as a percentage of negative control (untreated cells) proliferation. Values were expressed as mean ± SD of six experiments (p-value relative to control group: p <0.05).

Figure 2.  Antiproliferative effect of Origanum compactum Benth. extracts on MCF-7 cells. Cells were treated with various concentrations for 24 h (—), 48 h (-·-), and 72 h (…). (A) MCF-7 cells are treated by ethyl acetate extract. (B) MCF-7 cells are treated by methanol extract. Results are presented as percentage of negative control (untreated cells) proliferation. Values were expressed as mean ± SD of six experiments (p-value relative to control group: p <0.05).