Anti-inflammatory activity of essential oil from leaves of Myrciaria tenella and Calycorectes sellowianus

Figures & data

Table 1.  Constituents of the essential oil from leaves of Calycorectes sellowianus and Myrciaria tenella (Myrtaceae).

RI	Constituents	C. sellowianus	M. tenella
Monoterpene hydrocarbons		0.4	2.1
895	α-Pinene	0.1	1.5
934	β-Pinene	-	0.6
987	Limonene	0.3	-
Sesquiterpene hydrocarbons		28.3	54.8
1297	δ-Elemene	1.9	-
1333	α-Copaene	0.4	3.2
1342	β-Boubornene	1.4	1.0
1350	β-Elemene	2.7	2.2
1376	β-Caryophyllene	8.6	25.1
1384	β-Gurjunene	0.4	-
1392	Aromadendrene	0.3	1.0
1397	α-Guaiene	0.3	-
1407	α-Humulene	1.4	4.2
1414	allo-Aromadendrene	0.5	1.6
1431	γ-Muurolene	2.8	1.5
1435	Germacrene D	0.7	0.7
1440	β-Selinene	1.0	2.8
1449	Viridiflorene	1.6	-
1450	α-Selinene	-	4.4
1455	α-Muurolene	0.7	0.6
1459	Germacrene A	-	0.4
1465	β-Bisabolene		3.1
1468	γ-Cadinene	0.8	0.6
1477	δ-Cadinene	1.6	1.8
1510	Germacrene B	0.7	-
Oxygenated sesquiterpenes		71.3	43.3
1504	Elemol	0.6	0.4
1514	E-nerolidol	0.4	-
1522	Ledol	0.8	0.3
1537	Spathulenol	6.6	9.7
1539	Caryophyllene oxide	4.4	3.4
1543	Globulol	4.1	5.9
1551	Guaiol	13.1	1.0
1560	Eudesmol**	-	2.5
1562	Humulene oxide I	3.1	-
1567	Humulene epoxide II	0.4	0.5
1574	1-epi-Cubenol	0.5	1.3
1581	Eudesmol**	0.6	1.5
1595	Caryophylladienol II	-	0.6
1593	iso-Spathulenol	7.3	0.7
1601	τ-cadinol	-	1.8
1604	τ-Muurolol	6.3	-
1608	α-Muurolol	2.1	0.6
1611	β-Eudesmol	1.0	-
1618	α-Cadinol	7.9	5.2
1631	Calamenenol**	-	0.5
1647	Khusinol	0.6	-
	Total	87.8	92.1

*RI, linear retention indices in a DB-5 column. **isomer not identified.

Figure 1.  Chemical profile of essential oils from Calycorectes sellowianus and Myrciaria tenella leaves, obtained by GC-MS in a DB-5 column.

Table 2.  Chemotaxis inhibition of the essential oils of Myrciaria tenella and Calycorectes sellowianus treated cells.

Sample	Migrated distance (μm) ± MSE (% inhibition)
Control	119.0 ± 2.58
M. tenella (9 mg/mL)	8.0 ± 1.79* (93.3)
C. sellowianus (9 mg/mL)	10.28 ± 3.55* (91.4)
Genistein (108 µg/mL = 400 µM)**	9.9 ± 1.00 (91.7)

*Each value is the mean ± MSE. Student’s t-test: *P ≤ 0.005;**Positive control.

Figure 2.  Effect of pre-treatment with the essential oil of Myrciaria tenella and Calycorectes sellowianus (50 mg/kg, p.o., 1 h beforehand) on carrageenan-induced paw edema in rats. Indomethacin (10 mg/kg, p.o., 1 h beforehand) was used as positive control. Each point represents the mean of seven animals, and vertical lines show the MSE (Mean Standard Error). *The significance levels in comparison to control values: P < 0.05 (Student’s t-test).