Tegillarca granosa extract Haishengsu inhibits the expression of p-glycoprotein and induces apoptosis in drug-resistant K562/ADM cells

Figures & data

Table 1.  Haishengsu (HSS) induced apoptosis in the K562/ADM cells.

	Apoptosis (%)
	12 h	24 h	36 h
Control group	2.26 ± 1.26	2.25 ± 1.21	2.31 ± 1.24
HSS (10 µg/mL)	2.46 ± 1.28	3.68 ± 1.57	5.54 ± 2.54**
HSS (20 µg/mL)	4.51 ± 2.24*	5.36 ± 2.63*	9.35 ± 3.21**
HSS (40 µg/mL)	10.53 ± 2.45^§	20.69 ± 2.56^§	35.45 ± 3.21^§**

*P < 0.05 versus control group; **P < 0.01 versus 24 h and 12 h in the same group; § P < 0.01 versus control, HSS20 µg/mL and HSS 10 µg/ mL groups.

Figure 1.  Effect of Haishengsu (HSS) on the apoptosis of K562/ADM cells. There was an increase in apoptosis from lower to the higher HSS groups. (A) control group, (B) Haishengsu (10 μg/mL); (C) Haishengsu (20 μg/mL); (D) Haishengsu (40 μg/mL).

Table 2.  Optical density value of control and study groups.

	Control group	Haishengsu group (μg/mL)
		10	20	40
Caspase-3	79.6 ± 6.1	107.0 ± 5.4*	132.2 ± 4.4*	154.4 ± 5.0*
Bcl-2	104.6 ± 4.7	91.2 ± 3.7*	80.8 ± 3.8*	53.0 ± 5.2*

*P < 0.05 compared with the values in the control group.

Figure 2.  The expression of caspase-3 and Bcl-2 in the HSS and control (H0) groups. H1, H2 and H3 denote the concentration of HSS at 10, 20 and 40 μg/mL, respectively. There was a thickening in the 29 KD caspase-3 bands from H0 to H3 groups. There was a thinning in the 26 KD Bcl-2 bands.