Determination of escin content in androgenic embryos and hairy root culture of Aesculus hippocastanum

Figures & data

Figure 1.  Horse chestnut androgenic embryo at the cotyledonary stage of development cultured on MS solid phytoregulator-free medium.

Table 1.  Escin content in androgenic embryos and hairy root cultures of Aesculus hippocastanum.

Plant material	Phytoregulator	Content of aescin X (%) ± SD
Zygotic embryos		6.96 ± 0.1^a
Leaves		3.05 ± 0.02^gh
Globular embryos	Phytoregulator-free	0.59 ± 0.08^k
Cotyledonary embryos	Phytoregulator-free	2.43 ± 0.1^hi
	2,4-D	6.77 ± 0.45^ab
	NAA	6.03 ± 1.15^bc
	IBA	5.45 ± 0.35^cd
	Kin	5.34 ± 0.18^cd
	BA	4.66 ± 0.46^de
	ABA	6 ± 0.09^bc
Hairy root clones
47	Phytoregulator-free	4.09 ± 0.06^ef
39	phytoregulator-free	3.57 ± 0.03^fg
31	phytoregulator-free	1.9 ± 0.04^ij
43	phytoregulator-free	1.16 ± 0.01^jk

The escin content is expressed as percentages on the basis of dry weight. Data indicate means ± SD of three independent experiments (n = 9). Values marked with different letters are significantly different at a 0.05 probability level according to the protected LSD test.

Figure 2.  Hairy root culture of clone 43 in liquid MS medium.