Hypoglycemic activity of Ziziphus mauritiana aqueous ethanol seed extract in alloxan-induced diabetic mice

Figures & data

Figure 1.  Standardized chromatogram of seed extract of Ziziphus mauritiana. Chromatogram of seed extract of Ziziphus mauritiana employing HPLC profile of chemical marker. HPLC was performed using acetonitrile:water gradient over a period of 60 min: acetonitril 10%, 0.01–5 min; 10%–60%, 5–30 min; 60%–98%, 30–40 min; 98%, 40–45 min; 98%–10%, 45–55 min; 10%, 55–60 min. Other conditions were same as described in materials and methods.

Figure 2.  Effect of acute treatment of seed extract of Ziziphus mauritiana, glyburide and combination (seed extract and glyburide) on blood glucose level in alloxan-induced diabetes in mice. Blood glucose levels were assessed at regular interval of 0, 2, 4, 6 and 24 h after administration of extract at different concentration, glyburide and combination. The results are presented as mean ± SEM (n = 6). *p<0.001 compared with untreated control group.

Figure 3.  Effect of sub-acute treatment of seed extract of Ziziphus mauritiana, glyburide and combination (seed extract and glyburide) on blood glucose level in alloxan-induced diabetes in mice. Blood glucose levels were assessed on day 0, 7, 14, 21 and 28 after simultaneous administration of extract at different concentration, glyburide and combination. The results are presented as mean ± SEM (n = 6). *p <0.001 compared with untreated control group.

Figure 4.  Effect of treatment of seed extract of Ziziphus mauritiana, glyburide and combination (seed extract and glyburide) on blood glucose level in OGTT in diabetic mice. Blood was collected and assessed for blood glucose levels at 0. 30, 60, and 120 min after loading of glucose in diabetic mice. The results are presented as mean ± SEM (n = 6). *p <0.001 compared with untreated control group.

Figure 5.  Effect of treatment of seed extract of Ziziphus mauritiana, glyburide and combination (seed extract and glyburide) on blood glucose level in OGTT in normal mice. Blood was collected and assessed for blood glucose levels at 0, 30, 60, and 120 min after loading of glucose in normal mice. The results are presented as mean ± SEM (n = 6). *p<0.001 compared with untreated control group.

Table 1.  Effect of aqueous-ethanol seed extract of Ziziphus mauritiana on body weight (g) in alloxan-induced diabetic mice.

Dose (mg/kg)	Day 0	Day 7	Day 14	Day 21	Day 28
Control	31 ± 0.28	27 ± 0.91	23 ± 1.24	21 ± 0.12	18 ± 0.71
Glyburide (10 mg/kg)	30 ± 0.58	29 ± 0.63^*	31 ± 1.02^*	31 ± 0.28^*	31 ± 0.46^*
Seed extract (100 mg/kg)	29 ± 0.68	27 ± 0.12^*	26 ± 0.08^*	23 ± 0.49^*	20 ± 0.21^*
Seed extract (400 mg/kg)	30 ± 0.61	29 ± 0.17^*	29 ± 0.31^*	29 ± 0.36^*	29 ± 0.11^*
Seed extract (800 mg/kg)	29 ± 0.16	30 ± 0.42^*	30 ± 1.28^*	30 ± 1.32^*	30 ± 1.41^*
Combination	31 ± 0.44	32 ± 0.57^*	32 ± 1.18^*	32 ± 1.26^*	32 ± 0.85^*

The results are presented as mean ± SEM (n = 6), data were analyzed by one-way ANOVA followed by post hoc Tukey’s multiple range test. All the values were significant (p <0.001*) as compared with the vehicle-treated group (distilled water, 10 ml/kg).