Hypoglycemic and antioxidant effects of Rheum franzenbachii extract in streptozotocin-induced diabetic rats

Figures & data

Figure 1.  HPLC of ethanol extract from the roots and rhizomes of Rheum franzenbachii.

Figure 2.  Structure of stilbenes 1 and 2 isolated from the roots and rhizomes of Rheum franzenbachii.

Table 1.  Hypoglycemic effect of extract of Rheum franzenbachii (ERF) on plasma glucose levels (PGLs) in experimental rats.

Group	PGL (mg/dL)
	Before	After
Normal control	78.6 ± 2.5	80.2 ± 3.7
Diabetic control	347.5 ± 17.3*	355.4 ± 16.6
Metformin (500 mg/kg bw)	339.8 ± 16.7	101.5 ± 4.2**
ERF (125 mg/kg bw)	340.2 ± 14.9	248.4 ± 10.6**
ERF (250 mg/kg bw)	346.3 ± 16.2	143.0 ± 7.4**
ERF (500 mg/kg bw)	342.7 ± 15.7	117.1 ± 5.6**

Note. Values represent mean ± SEM. *p < 0.01 compared with normal control group. **p < 0.01 compared with diabetic control group.

Figure 3.  Effect of extract of Rheum franzenbachii (ERF) on plasma glucose levels (PGLs) during oral glucose tolerance test in streptozotocin (STZ)-induced diabetic rats. *p < 0.01 compared with initial value, **p < 0.01 compared with value of 30 min.

Figure 4.  Values of area under the curve (AUC) from basal level at time 0 during the course of 120 min in STZ-induced diabetic rats. *p < 0.01 compared with diabetic control group.

Table 2.  Antioxidant effect of ERF on MDA, GSH, SOD, and CAT in experimental rats.

Group	0 days				14 days
	MDA (μmol/g Hb)	GSH (μmol/g Hb)	SOD (U/g Hb)	CAT (U/g Hb)	MDA (μmol/g Hb)	GSH (μmol/g Hb)	SOD (U/g Hb)	CAT (U/g Hb)
NC	1.77 ± 0.31	4.59 ± 0.42	0.28 ± 0.05	1.13 ± 0.16	1.79 ± 0.33	4.62 ± 0.64	0.29 ± 0.03	1.14 ± 0.17
DC	3.70 ± 0.44*	2.35 ± 0.37*	0.17 ± 0.04*	0.67 ± 0.14*	4.25 ± 0.47	2.13 ± 0.50	0.14 ± 0.02	0.62 ± 0.11
Metformin (500 mg/kg)	3.63 ± 0.41	2.31 ± 0.47	0.16 ± 0.03	0.69 ± 0.16	1.97 ± 0.33**	4.16 ± 0.58**	0.27 ± 0.05**	1.02 ± 0.14**
ERF (125 mg/kg)	3.59 ± 0.39	2.27 ± 0.43	0.19 ± 0.05	0.66 ± 0.12	2.94 ± 0.30**	2.98 ± 0.45**	0.23 ± 0.04**	0.74 ± 0.15
ERF (250 mg/kg)	3.74 ± 0.40	2.34 ± 0.39	0.17 ± 0.02	0.65 ± 0.13	2.33 ± 0.37**	3.62 ± 0.52**	0.26 ± 0.03**	0.91 ± 0.12**
ERF (500 mg/kg)	3.68 ± 0.35	2.30 ± 0.42	0.16 ± 0.05	0.66 ± 0.12	2.06 ± 0.32**	3.97 ± 0.49**	0.28 ± 0.06**	1.00 ± 0.14**

Note. Values represent mean ± SEM. *p < 0.05 compared with normal control (NC) group. **p < 0.05 compared with diabetic control (DC) group.