Immunostimulant activity of noni (Morinda citrifolia) on T and B lymphocytes

Figures & data

Figure 1.  Effect of extracts and fractions of Morinda citrifolia fruits on splenocyte proliferation. Numbers written above bar graphs indicate concentrations in mg mL⁻¹ of M. citrifolia fruit extractives, namely aqueous and hydroalcoholic extracts, fractions F I, F II, and F III. *, A slight and insignificant decrease in proliferation of isolated splenocytes; **, a significant increase in proliferation of isolated splenocytes.

Table 1.  Effect of administration of extracts and fractions of fruits of Morinda citrifolia on cell mediated immune response in rats.

Treatment	Dose (mg kg^−1)	Percent increase in paw volume (mean ± SE)
Vehicle control I	—	10.01 ± 2.89
Vehicle control II	—	2.39 ± 0.43*
Hydroalcoholic extract	50	9.54 ± 1.15
	100	16.73 ± 2.66
	200	33.52 ± 4.93*
Aqueous extract	50	3.75 ± 1.44
	100	6.68 ± 0.49
	200	18.56 ± 2.29*
Fraction I	10	5.02 ± 1.77
	20	9.09 ± 2.9
	40	13.89 ± 0.71
Fraction II	0.25	1.67 ± 0.38
	0.5	4.58 ± 0.42
	1.0	11.60 ± 0.84
Fraction III	0.05	4.85 ± 0.88
	0.1	7.53 ± 0.67
	0.2	15.43 ± 1.03
Withania somnifera	100	21.82 ± 1.1*

Note. n = 6. All groups with the exception of vehicle control group I were co-administered prednisolone (5 mg kg⁻¹) to induce immunosuppression. *p < 0.05 statistically significant as compared to vehicle control I (normal animals).

Table 2.  Effect of administration of extracts and fractions of fruits of Morinda citrifolia on anti-sheep red-blood cell (SRBC) antibody titer in rats.

Treatment	Dose (mg kg^−1)	Anti-SRBC antibody titer (mean ± SE)	% Change in anti-SRBC antibody titer
Vehicle control I	—	8.0 ± 1.15	—
Vehicle control II	—	5.0 ± 0.58*	−60.0
Hydroalcoholic extract	50	6.67 ± 0.67	−19.94
	100	8.67 ± 0.88	+7.73
	200	12.0 ± 1.15*	+33.33
Aqueous extract	50	5.67 ± 0.33	−41.09
	100	6.33 ± 0.33	−26.38
	200	8.67 ± 0.33	+7.73
Fraction I	10	6.33 ± 0.33	−26.38
	20	8.33 ± 1.24	+3.96
	40	12.33 ± 1.21*	+35.12
Fraction II	0.25	6.0 ± 0.88	−33.33
	0.5	7.33 ± 0.88	−9.14
	1.0	7.67 ± 1.22	−4.30
Fraction III	0.05	5.67 ± 0.33	−41.09
	0.1	6.33 ± 1.33	−26.38
	0.2	6.67 ± 1.45	−19.94
Withania somnifera	100	12.33 ± 0.88*	+35.12

Note. n = 6. All groups with the exception of vehicle control group I were co-administered prednisolone (5 mg kg⁻¹) to induce immunosuppression. *p < 0.05 statistically significant as compared to vehicle control I (normal animals).