Figures & data
Figure 1. Cytotoxic effects of the six enhancers on Caco-2 cell monolayers. Cytotoxicity was measured by the MTT assay following 2, 6, and 24 h incubation at 37°C, 5% CO2 and 95% relative humidity. For the MTT assay, PBS and DMSO were used as positive (100% cell viability) and negative (0% cell viability) controls, respectively. All measurements were expressed as mean ± SD.
Figure 2. Cumulative permeation of EFEs across the Caco-2 cell monolayers. Six different enhancers were added to the apical compartment of the insert individually to increase the EFEs permeation.
Figure 3. FITC-labeled EFEs and different enhancers were simultaneously intragastrically administrated to mice. After 2, 6, and 24 h, serum samples were obtained and counted by Bio-Tek fluorescence spectrophotometer.
Figure 4. Light micrographs of microvilli from duodenum epithelia tissues at 24 h after oral administration of the enhancers in healthy mice (×400).
