Neuroprotective effect of Hibiscus rosa sinensis in an oxidative stress model of cerebral post-ischemic reperfusion injury in rats

Figures & data

Table 1.  Effects of H. rosa sinensis root extract on bilateral common carotid artery occlusion-induced alterations in rat brain CAT, SOD, LPO and GSH.

Treatment(mg/kg)	CAT (µmol of H2O2 decomposed/min/mg protein)	SOD (U / mg protein)	LPO (nmol of MDA/mg protein)	GSH (µmol of GSH/mg protein)
Control	9.3 ± 0.5	5.4 ± 2.4	3.4 ± 1.0	4.0 ± 0.2
Vehicle	4.6 ± 0.2^a**	0.5 ± 0.1	23.8 ± 3.0^a**	1.4 ± 0.1^a**
HRS − 100	6.9 ± 0.2^b**	2.4 ± 0.3	12.6 ± 1.1^b**	2.2 ± 0.04^b**
HRS − 200	7.3 ± 0.1^b**	3.9 ± 0.7	11.0 ± 1.1^b**	3.0 ± 0.1^b**
HRS − 300	7.6 ± 0.2^b**	5.02 ± 2.4	11.7 ± 1.7^b**	3.2 ± 0.05^b**

Values are expressed as mean ± SEM (n = 5). ^aCompared with the control-treated group; ^bCompared with the vehicle-treated group. **p < 0.01 (one way ANOVA followed by Dunnett’s test).

Table 2.  Effects of H. rosa sinensis root extract (HRS) on bilateral common carotid artery occlusion (BCCAO)-induced alterations in open field parameters.

Treatment (mg/ kg)	Ambulations (Number)	Immobility (s)	Rearing (Number)	Grooming (Number)
Control	21.2 ± 2.3	107.2 ± 8.4	19.2 ± 2.6	9.2 ± 1.2
Vehicle	6.8 ± 0.8^a**	224.0 ± 8.9^a**	4.2 ± 0.4^a**	2.8 ± 0.7^a**
HRS - 100	9.0 ± 1.6	206.2 ± 4.1	5.4 ± 1.2	3.4 ± 0.6
HRS - 200	13.6 ± 2.2	176.6 ± 12.0^b**	8.8 ± 1.4	3.8 ± 0.3
HRS - 300	14.8 ± 1.8^b*	183.2 ± 5.3^b**	12.2 ± 2.1^b*	6.2 ± 0.8^b*

Values are expressed as mean ± SEM (n = 5). ^aCompared with control-treated group; ^bCompared with vehicle-treated group. *p < 0.05; **p < 0.01 (one-way ANOVA followed by Dunnett’s test).

Figure 1.  Effect of H. rosa sinensis root extract on learning and memory in bilateral common carotid artery occlusion-induced alterations of transfer latency at day 0, 1 and 6 in the elevated plus maze. Each column represents mean ± SEM (n = 5). ^aCompared with control-treated group. ^bCompared with vehicle-treated group. *p < 0.05; **p < 0.01 (one-way ANOVA followed by Dunnett’s test).