Chemical composition and antibacterial activity of essential oils of Tripleurospermum disciforme in three developmental stages

Figures & data

Table 1.  Chemical composition of aerial parts of Tripleurospermum disciforme prepared in three developmental stages. Amounts were expressed as percentage of dry weight.

	Compound	Prior to flowering	During flowering	After flowering
1	Alloaromadendrene	0.43	0.6	0.75
2	Pentadecane	0.75	0.45	0.5
3	Cycloisolongifolene	0.5	0.62	0.4
4	β-Ylangene	0.72	0.92	0.56
5	δ-Cadinene-δ-guaiene	2.2	1.74	1.5
6	β-Farnesene	19.24	22.46	18.3
7	Dodecane	0.2	1.1	1.96
9	Unknown	1.35	0.68	0.56
10	β-Caryophyllene	1.65	0.5	0.38
11	Unknown	2.13	1.94	1.65
12	Benzene acetaldehyde	6.5	9.3	7.7
13	Spathulenol	0.93	1.35	1.48
14	Unknown	1.45	Unknown	1.26
15	Caryophyllene oxide	0.92	1.36	1.14
16	p-methoxy-Humulene oxide	8.5	6.88	4.75
17	Unknown	0.49	0.1	0.4
18	β-Eudesmol	0.81	1.25	2.86
19	Heptadecane	9.8	10.6	9.65
20	β-Sesquiphellandrene	15.4	17.85	12.78
21	p-methoxy-β-Cyclopropylstyrene	16.94	16.64	13.85
	Total	90.91	97.74	82.43

Table 2.  Antimicrobial activity of the essential oil of T. disciforme that was expressed as diameter of inhibition zone (mm).

Bacterial strains	Prior to flowering	Flowering	After flowering	Chloramphenicol	MIC
					EO^a (µL/mL)	STD^b (µg/mL)
Citrobacteramalonaticus	12 ± 4	16.5 ± 4	13 ± 4	13 ± 4	22	8
Proteusvulgaris	14 ± 3	18 ± 3	15 ± 3	35 ± 8	20	4
Serratiamarcescens	14 ± 6	17 ± 3	16 ± 4	22 ± 5	20	4
Enterobacteraerogenes	18 ± 4	22 ± 4	18 ± 6	22 ± 3	8	4
Staphylococcusaureus	22 ± 5	28 ± 6	25 ± 7	25 ± 3.5	4	4
Staphylococcussubtilis	12 ± 2	15 ± 4	14 ± 2	25 ± 4	20	4
Bacillusmegaterium	18 ± 4	22 ± 6	19 ± 3	18 ± 2.5	8	1
Bacillus cereus	21 ± 4	25 ± 2	25 ± 5	17 ± 3	4	4

^aEssential oil.

^bChloramphenicol standard.

Each datum represents the means ± SE of 4-5 samples.