Figures & data
Figure 1. Inhibitory activity of Gardenia jasminoids Ellis extract on human DNA topoisomerase I (A). Lane a, 0.1 μg Supercoiled DNA without added enzyme; lane b, supercoiled DNA with 1 U DNA topoisomerase 1; lanes c-h, supercoiled DNA with 1 U DNA topoisomeraise 1 in the presence of (lane c) n-hexane, (lane d) dichloromethane, (lane 3) ethyloxylacetate, (lane f) n-butanol, (lane g) water fractions, and (lane h) methanol extract of gardenia. Cytoxic effects (B) of gardenia fractionated methanol extract on KB cells. MeOH, methanol; HX, hexane; DCM, dichloromethane; EtOAc, ethyloxylacetate; BuOH, butanol; and H2O, water fractions. A comparison of the cytoxic effects of gardenia methanol extract (C) dichloromethane fraction, (D) on normal oral keratinocyte HaCat cells and oral cancer KB cells. The vertical bars indicate the means ± SD (n = 3). Significant difference at *P < 0.05 was compared to control.
Figure 2. Cytoxic effects of gardenia dichloromethane fraction on KB cells. Cells viability, (A) histomorphologic evaluation, (B) and flow cytometric analysis (C) in KB cells treated with gardenia dichloromethane fraction. Cells were treated with 0.1 mg/ml dichloromethane extract for the indicated times and analyzed. All magnifications are × 200.
Figure 3. Effect of dichloromethane fraction on capase activities and PARP protein expression. Caspase-3, caspase-8, and caspase-9 activity (A) and Poly (ADP ribose) polymerase (PARP) protein expression (B) by treatment of dichloromethane fraction on KB cells. Actin was used to ensure equal protein loading.
