Antioxidant, antihyperuricemic and xanthine oxidase inhibitory activities of Hyoscyamus reticulatus

Figures & data

Figure 1.  (A) Concentration-response curve for the absorbance at 405 nm for ABTS^•+ as a function of standard Trolox solution. Data are expressed as mean of three replicates ± SEM. (B) ABTS^•+ scavenging capacities of five concentrations of H. reticulatus aqueous extract. Data are expressed as the mean of three replicates.

Figure 2.  Effects of H. reticulatus aqueous extract and allopurinol on serum urate levels in the hyperuricemic mice pretreated with the potassium oxonate. Data represent mean values (± SEM) of urate levels in serum (mg/dL) in the groups of animals (n = 5). ^#P <0.001 vs. normal control group, *P <0.01, **P <0.001 versus hyperuricemic control group.

Table 1.  Average serum urate levels of oxonate-Balb/c mice after oral administration of three doses of H. reticulatus extract concentrations (200, 400 and 600 mg/kg).

Treatment	Dose (mg/kg)	Serum uric acid (mg/dL)
Normal animal	−	1.520 ± 0.183
Hyperuricemic animal
vehicle	−	4.770 ± 0.515^#
H. reticulatus	200	1.680 ± 0.456*
	400	0.739 ± 0.297**
	600	0.387 ± 0.093**
Allopurinol	20	0.740 ± 0.279**

Data represents mean ± SEM of five animals. For statistical significance, Student’s t-test was used between control and drug groups.

^#P <0.001 (compared to no-treatment control group); *P <0.01 (compared to hyperuricemia control group); **P <0.001 (compared to hyperuricemia control group).

Figure 3.  Concentration-response curve for the absorbance at 765 nm for gallic acid standard. Data are expressed as mean of three replicates ± SEM.

Figure 4.  The inhibitory effect of H. reticulatus aqueous extract concentrations on the activity of XO. Data are expressed as the mean of three replicates ± SEM.