Protective effect of partially purified 35 kDa protein from silk worm (Bombyx mori) fecal matter against carbon tetrachloride induced hepatotoxicity and in vitro anti-viral properties

Figures & data

Table 1.  Effect of protein on CCl₄ induced hepatotoxicity in rats.

Group	Treatment	AST	ALT	ALP	Total bilirubin
Group I	Control	242.02 ± 18.39	139.38 ± 14.6	456.08 ± 25.12	0.44 ± 0.03
Group II	CCl4	1400.05 ± 78.82***	1060.47 ± 65.67***	963.48 ± 55.22***	2.45 ± 0.16***
Group III	Protein (50 mg) + CCl4	666.9 ± 43.04**	538.27 ± 67.88**	589.47 ± 34.85**	1.59 ± 0.09**
Group IV	Protein (100 mg) +CCl4	339.5 ± 24.3**	236.6 ± 31.7**	541.4 ± 32.68**	1.19 ± 0.05**

Values are the mean ± SEM of six rats; ***P <0.001 significant compared to control; **P <0.01 significant compared to CCl₄-intoxicated control.

Figure 1.  Radical scavenging activity of partially purified protein by DPPH method.

Figure 2.  Superoxide anion scavenging activity of partially purified protein.

Figure 3.  Effect of partially purified protein against CCl₄-induced histological liver injury: (A) normal control rats showing hepatic cells with well-preserved cytoplasm (H), well brought out central vein (CV) and prominent nucleus and nucleolus; (B) liver section of CCl₄-treated rats showing massive fatty changes (F), necrosis (NC), and the loss of cellular boundaries; (C) liver section of rats treated with CCl₄ and protein 100 mg/kg bw, retaining normal hepatic architecture with fewer areas of fatty change, and necrosis.