Figures & data
Figure 1. Effects of SbG on HUVE cell viability and cytotoxic effect of acrolein on HUVE cells. The cells were treated with SbG or acrolein for 24 h at appropriate concentrations. MTT was conducted for the cell viability assay. Data were presented as mean±SEM of results in three independent experiments. Each experiment was performed in triplicate. *P< 0.05, **P <0.001 versus control (black bar).
Figure 2. Protective effects of SbG pretreatment at 50 and 100 μg/ml showing the protection against acrolein-induced cytotoxicity in the HUVE cells. Cells were plated in the 96-well plate and SbG at different concentrations were loaded into the appropriate wells and incubated for 24 h. The media was discarded and the cells were continuously cultured in the presence or absence of acrolein for another 24 h. MTT was conducted for cell viability. Data were expressed as mean±SEM of the results of three independent experiments. *P < 0.05, **P < 0.01 vs acrolein alone groups.
Figure 3. Effects of SbG on cellular GSH levels and GST activity. HUVE cells were incubated with 50 and 100µg/ml SBG for 24 h. GSH contents were evaluated (*P < 0.05 vs the control). However, no significant GST activity changes were observed in the SbG treated when compared with the control group. Data were presented as mean ±SEM of the results of three independent experiments.
Figure 4. Protective effects of SbG on HUVE cells. The cells were treated with acrolein for 24 h at different concentrations. In the another set of the experiment, the cells were pretreated with 100 µg/mL SbG. Data were presented as mean±SEM of three independent experiments. *P <0.05, ** P < 0.01 versus the control, # P < 0.05, versus the acrolein alone group.
Figure 5. After 24 h pretreatment of 100 µg/mL SbG, mRNA levels of both GCLC and GCLM were elevated. Acrolein (40 µM) treatment inhibited the SbG induced GCLC mRNA up-regulation levels. Data were mean±SEM of three independent experiments. *P <0.05, ** P < 0.01 versus the control; # P < 0.05, versus the SBG alone group.
