Anticonvulsant activity of solasodine isolated from Solanum sisymbriifolium fruits in rodents

Figures & data

Figure 1.  GC-MS analysis of solasodine isolated from S. sisymbriifolium. The GC-MS graph shows base peak at 114 m/z (A); the intense fragment peak at 138 m/z (B); and molecular weight of solasodine at 413 m/z (C).

Table 1.  Effect of solasodine on picrotoxin-induced convulsions in mice.

Group	Dose (mg/kg, i.p.)	HLTE^† Latency (min)	% survival
Control	-	16.3 ± 2.2	0
DZP	4	45.0 ± 0.0**	80
Solasodine	25	29.9 ± 2.7*	0
Solasodine	50	20.3 ± 1.8	0
Solasodine	100	22.4 ± 3.2	0

^†Values are expressed as mean ± SEM (n = 6).

*p <0.01; **p <0.001, as compared to control group (one-way ANOVA followed by the Tukey test).

Table 2.  Effect of solasodine on pentylenetetrazole-induced convulsion test in mice.

Group	Dose (mg/kg, i.p.)	Latency of HLTE^† (min)	% of survival
Control	–	13.4 ± 0.3	0
DZP	4	45.0 ± 0.0**	80
Solasodine	25	15.5 ± 1.2^ns	0
Solasodine	50	6.5 ± 0.7^ns	0
Solasodine	100	12.1 ± 2.0^ns	0

^†Values are expressed as mean ± SEM (n = 6).

^nsnot significant.

**p <0.001, as compared to control group (one-way ANOVA followed by Tukey test).

Table 3.  Effect of solasodine on maximal electroshock-induced convulsions in rats.

Group	Dose (mg/kg, i.p.)	Duration of HLTE^† (s)	% of survival
Control	–	11.0 ± 1.0	100
PHE	25	5.0 ± 0.0**	100
Solasodine	25	7.4 ± 0.4**	100
Solasodine	50	6.2 ± 0.3**	100
Solasodine	100	2.4 ± 0.2**	100

^†Values are expressed as mean ± SEM (n = 6).

**p <0.001 as compared to control group (one-way ANOVA followed by Tukey test).

Figure 2.  Effect of solasodine in thiopental-provoked sleep time. The data are presented as mean ± SEM of six animals. *p <0.05, ***p <0.001, as compared to control group (one-way ANOVA followed by the Tukey test).