Cell line studies of glioblastoma and malignant glioma using a newly discovered prenylated chalcone

Figures & data

Table 1.  Experimental data and the observed response values (normalized cell viability, %) of CNS1 and 13-06 cells with two independent factors, X₁ (prenylated chalcone concentration, μg/ml) and X₂ (uptake time, hours).

Run No.	Factors		Response values Normalized cell viability (%)
	X1 (μg/ml)	X2 (hours)	CNS1	13-06
1	10 (−1)^a	12 (−1)	−5.6	−23.5
2	10 (−1)	36 (1)	−17.2	−40.3
3	20 (1)	12 (−1)	−11.3	−26.2
4	20 (1)	36 (1)	−19.1	−52.4
5	15 (0)	24 (0)	−5.8	−27.4
6	15 (0)	24 (0)	−9.2	−26.7
7	15 (0)	24 (0)	−9.9	−26.8
8	15 (0)	24 (0)	−5.3	−27.7
9	15 (0)	24 (0)	−4.6	−27.2
10	22.1 (1.41)	24 (0)	−50.4	−62.8
11	7.9 (−1.41)	24 (0)	−9.3	−17.3
12	15 (0)	41 (1.41)	−20.9	−7.2
13	15 (0)	7 (−1.41)	−23.5	−19.8

^a (−1), (0), and (1) are coded symbols for levels of independent factors.

Response values were means of three replicates.

Figure 1.  The general chemical structure of a typical prenylated chalcone compound.

Table 2.  Regression coefficients of the second-order model for CNS1 cells.

Regression coefficient	Estimate	t Value	Pr > | t |
Intercept β0	−6.9615	−1.52	0.1720
β1	−8.2157	−2.27	0.0574
β2	−1.9657	−0.54	0.6038
β11	−8.2640	−2.13	0.0707
β12	0.9500	0.19	0.8580
β22	−4.4379	−1.14	0.2904

Table 3.  Regression coefficients of the second-order model for 13-06 cells.

Regression coefficient	Estimate	t Value	Pr > | t |
Intercept β0	−64.0768	−1.15	0.2872
β1	9.2499	1.61	0.1514
β2	−1.2093	−0.58	0.5774
β11	−0.3429	−1.98	0.0885
β12	−0.0392	−0.41	0.6950
β22	0.0320	1.06	0.3249

Figure 2.  Response surface plot for the use of prenylated chalcone in the cell line study of CNS1 (glioblastoma) cells.

Figure 3.  Response surface plot for the use of prenylated chalcone in the cell line study of 13-06 (malignant glioma) cells.