Figures & data
Figure 1. Scavenging of free radicals and reducing activities of H. multiflorum extract. Scavenging activities are expressed as micromole equivalents of Trolox per milligram of dry matter. Reducing activity is expressed as micromole equivalents of ascorbic acid per milligram of dry matter. The values represent the mean ± SE (n = 5).
Figure 2. Inhibition of lipid peroxidation by H. multiflorum extract. Peroxidation was induced by treatment with 10 µM FeSO4 and 100 µM ascorbic acid for rat brain homogenate and 1 mM CuSO4 for human plasma. Each value represents the mean ± SD (n = 5). IC50 values were obtained from the concentration/effect regression line.
Figure 3. Promotion of PMN cells apoptosis by H. multiflorum extract (T). (A) Apoptosis was measured by three distinct methods: analysis of apoptotic (hypodiploid) nuclei, measurement of phosphatidylserine exposure, and quantitation of uptake and retention of DIOC6(3). All values are represented as means ± SD of four separate experiments. (B) Typical histograms showing the percentage (M1) of nuclei with hypodiploid DNA content. (C) Representative dot plots of Annexin V/PI staining are shown. The lower left quadrant contains the vital (double negative) population. The lower right quadrant contains the apoptotic (Annexin V+/PI+) population. Finally, cells in the top right quadrant (Annexin V+/PI+) are in later stages of apoptosis and necrosis. (D) PMNs were loaded with DIOC6(3) and analyzed by FACS.
