Figures & data
Figure 1. Cytotoxicity of J-01 on RAW264.7 macrophage. RAW264.7 cells were incubated for 24 h with different con of J-01, ethanol extract of J. gendarussa. Cell viability was determined by (A) MTT assay and (B) SRB assay. Data are expressed as percentage of control. n = 3.
Figure 2. Effect of J-01 on LPS induced NO production from RAW264.7 cells. NO production was determined as nitrite accumulation in the medium after 24 h of drug treatment. Data represent µM of nitrite produced.*P < 0.01, n = 3.
Figure 3. The effect of J-01 extract on iNOS gene expression. RAW264.7 cells were stimulated with or without LPS (1 μg/mL).RNA was isolated from drug treated and untreated cells and RT-PCR was performed using specific primers as described in the text (A) iNOS and GAPDH mRNA (B) relative level of iNOS gene expression normalized to GAPDH RNA and values depict arbitrary units. Data is representative of three experiments. *P < 0.05.
Figure 4. The effect of J-01 extract on MMP-9 gene expression. RAW264.7 cells were stimulated with or without LPS (1 μg/mL). RNA was isolated from drug treated and untreated cells and RT-PCR was performed using specific primers as described in the text. (A) MMP-9 and GAPDH mRNA (B) relative level of MMP-9 gene expression normalized to GAPDH RNA and values depict arbitrary units. Data is representative of three experiments. *P < 0.05.
