Sedative and anticonvulsant activities of styrax after oral and intranasal administration in mice

Figures & data

Table 1.  Effect of styrax after oral and nasal administration on the onset and duration of sleep in mice induced by hypnotic dosage of pentobarbital (n = 10).

Groups	Dose (mg/kg)	Number of falling sleep	Sleep latency (min)	Sleeping time (min)
Control		10	4.3 ± 1.2	39.7 ± 0.75
Styrax i.n.	12.5	10	4.7 ± 2.0	72.0 ± 20.7**
Styrax i.n.	25	10	4.8 ± 1.3	53.7 ± 12.8*
Styrax i.n.	50	10	5.4 ± 1.0	52.1 ± 12.3*
Styrax i.g.	25	10	4.6 ± 2.0	76.4 ± 19.6**
Styrax i.g.	50	10	4.4 ± 1.5	55.7 ± 6.7*
Styrax i.g.	100	10	3.6 ± 1.3	47 ± 2.0*
Diazepam i.g.	3	10	4.2 ± 0.7	72.8 ± 27.4**

Mice received pentobarbital (40 mg/kg, i.p.) 1 min after the pretreatment of styrax (12.5, 25, 50 mg/kg, i.n.), (25, 50, 100 mg/kg, i.g.), and diazepam (3 mg/kg, i.g.). If mouse did not lose righting reflex in 1 h after injecting pentobarbital, the sleep latency and the sleeping time were recorded 60 and 0 min, respectively. Sleep latency and time were expressed as the means ± SD (n = 10). *P < 0.05, **P < 0.01, significant as compared with the control group. i.n., intranasal administration; i.g., intragastric administration.

Table 2.  Effect of styrax after oral and nasal administration on convulsions induced by pentylenetetrazol (PTZ) (n = 10).

Groups	Dose (mg/kg)	Latency time (min)	Mortality rate (%)	Tonic clonic convulsions rate (%)
Control		4.7 ± 2.26	60	100
Styrax i.n. 5 min	12.5	2.45 ± 0.12	40	100
Styrax i.n. 5 min	25	2.90 ± 2.92	30	100
Styrax i.n. 5 min	50	15.3 ± 7.6**	10	90
Styrax i.g. 30 min	50	2.3 ± 1.8	100	100
Styrax i.g. 30 min	100	3.6 ± 3.2	20	100
Styrax i.g. 30 min	200	7 ± 12.1	20	80
Diazepam 30 min	5	30	0	0

Effects of styrax in the incidence of clonic convulsions, the mortality rate and the latency of clonic convulsions induced by PTZ (90 mg/kg). *P < 0.05, **P < 0.01, significant as compared with the control group. i.n., intranasal administration; i.g., intragastric administration.

Figure 1.  Effect of styrax on spontaneous locomotor activity of mice after oral administration. Styrax (25, 50, 100 mg/kg) or vehicle (3% Tween 80) was administered. Each mouse was determined for 1 h. Data were expressed as number of movements (5–60 min) for 10 mice for each group. Each column represents mean of 10 mice.