Evaluation of antioxidant and free-radical scavenging potential of Artemisia absinthium

Figures & data

Table 1.  Superoxide anion radical scavenging activity of MAB and GA by the PMS-NADH-NBT method.

Sample	Concentration (µg/mL)	% Inhibition
MAB	25	19.40 ± 1.02
	50	43.01 ± 1.09
	75	69.50 ± 1.03^*
	100	78.12 ± 0.98^*
GA	50	65.05 ± 1.01
	75	82.12 ± 1.08
	100	91.60 ± 1.33

Values are presented as mean ± SD.

^*p < 0.05 compared to standard drug. Statistical analysis was done by using Student’s t-test.

GA, gallic acid; MAB, methanol extract of Artemisia absinthium.

Table 2.  Nitric oxides (NO) scavenging and hydrogen peroxide (H₂O₂) scavenging effects of MAB, BHA and α-tocopherol.

Sample	Concentration (µg/mL)	% Inhibition (NO)	% Inhibition (H2O2)	EC50^NO (µg/mL)	EC50^H2^O2 (µg/mL)
MAB	25	14.40 ± 1.08	15.07 ± 1.99	180.71	106.41
	50	21.21 ± 1.05	26.04 ± 1.06
	75	—	40.31 ± 1.00
	100	35.32 ± 0.98	47.98 ± 0.91
	200	56.22 ± 1.02	—
BHA				26.51	—
α-Tocopherol				—	18.52

BHA, butylated hydroxyanisole; MAB, methanol extract of Artemisia absinthium.

Figure 1.  Hydroxyl radical scavenging activity of MAB and BHT (25–100 µg/mL). BHT, butylated hydroxytoluene; MAB, methanol extract of Artemisia absinthium.

Figure 2.  Reducing power of MAB and BHT (50–400 µg/mL). BHT, butylated hydroxytoluene; MAB, methanol extract of Artemisia absinthium.

Figure 3.  Effect of methanol extract of Artemisia absinthium (MAB) on mitochondrial and supernatant thiobarbituric acid reactive substances formation in mice subject to global cerebral ischemia followed by reperfusion. Each column represents the mean ± SD, n = 7; a = p < 0.05 versus sham; b = p < 0.05 versus control; c = p < 0.05 versus 100 mg/kg, p.o., of the extract. TBARS, thiobarbituric acid reactive substances.

Table 3.  Effect of methanol extract of Artemisia absinthium (MAB) on superoxide dismutase (SOD) activity and reduced glutathione (GSH) content during cerebral post-ischemic reperfusion (15 min BCAO and 24-h reperfusion).

Group	SOD (U/mg protein)	GSH (nM/mg protein)
Sham	100.08 ± 14.11	5.59 ± 0.29
Control	63.50 ± 16.29^**	2.24 ± 0.71^**
MAB (100 mg/kg)	82.15 ± 7.45^***	3.70 ± 0.45^***
MAB (200 mg/kg)	95.99 ± 10.42^**	5.60 ± 0.55^**

All data are expressed as mean ± SD, n = 7. Sham and treatment groups are compared with control. Superscripts ^** and ^*** indicate p values < 0.01 and < 0.05, respectively. Statistical analysis was done by one way analysis of variance followed by Tukey’s Test.