An effective way to biosynthesize α-glucosyl eugenol with a high yield by Xanthomonas maltophilia

Figures & data

Figure 1.  The biocatalytic synthesis of eugenol α-glucoside by X. maltophilia BT-112 with maltose as a glucosyl donor.

Table 1.  ¹³C-NMR and ¹H-NMR data of α-EG in DMSO^a.

No.	δH	δC	No.	δH	δC
1		145.2	(Glc)1′	5.30 (1H, d, J = 1.86)	99.4
2		150.5	2′		72.2
3	6.801 (1H, s)	113.9	3′		74.1
4		134.7	4′		70.3
5	6.68 (1H,brd, J = 7.8)	121.0	5′		73.4
6	7.06 (1H, d, J = 7.8)	118.2	6′		61.0
7	3.75 (3H, s)	56.4
8	3.20 (2H, m)	39.9
9	5.94 (1H, m)	138.3
10	5.04 (2H, m)	116.1

^aδ in ppm and J in Hz. Run at 600 MHz for ¹H-NMR and 150 MHz for ¹³C-NMR.

Figure 2.  The HPLC analysis of pure eugenol (A) and reaction mixture (B) at 48 h; peak a, eugenol; peak b, α-EG.

Figure 3.  Effects of reaction temperature (A), shaking speed (B), pH (C), eugenol concentration (D), reactant composition (E), and reaction time (F) on eugenol conversion.