Fractionation of stigmasterol derivative and study of the effects of Celsia coromandelina aerial parts petroleum ether extract on appearance of puberty and ovarian steroidogenesis in immature mice

Figures & data

Figure 1.  Effects of petroleum ether extract of aerial parts of C. coromandelina (PECC) on content of ascorbic acid, cholesterol and the activities of G 6-PDH and D⁵-3b- HSD in mouse ovary. Results presented as mean ± SEM from 6 animals. Statistical analysis was done by ANOVA followed by post hoc test of significance, Dunnett’s t-test. *P < 0.05 as compared with vehicle control. PECC: petroleum ether extract of aerial parts of C. coromandelina. E estra: Ethinyl estradiol.

Table 1.  Mean age in days of female mice at two measures of sexual maturity and rate of body growth after treatment with petroleum ether extract of aerial parts of C. coromandelina (PECC).

Treatment with dose	Vaginal opening (days)	First estrus (days)	Initial body weight (g)	Final body weight (g)
Saline	52.1 ± 0.4	81.3 ± 0.8	10.1 ± 0.8	24.0 ± 1.0
(5 mL/kg, i.p.)
Vehicle (Arachis oil)	55.2 ± 0.8	81.9 ± 0.8	10.2 ± 0.7	23.9 ± 0.8
(5 mL/kg, i.p.)
Ethinyl estradiol	117.5 ± 1.9*	142.8 ± 2.0*	10.8 ± 0.8	23.0 ± 0.7
(0.03 mg/kg, i.p.)
PECC	72.0 ± 0.9*	97.1 ± 1.1*	10.9 ± 0.6	22.9 ± 0.6
(300 mg/kg, i.p.)
PECC	83.2 ± 1.0*	104.7 ± 0.9*	10.7 ± 0.4	22.6 ± 0.5
(400 mg/kg, i.p.)
PECC	85.7 ± 1.1*	109.3 ± 0.8*	11.0 ± 0.7	22.8 ± 0.6
(500 mg/kg, i.p.)
Ethinyl estradiol	179.1 ± 2.9**	204.7 ± 3.1**	10.2 ± 0.8	23.0 ± 0.8
(0.03 mg/kg, i.p.)
+PECC (300 mg/kg, i.p.)

Results presented as mean ± SEM from 6 animals. Vehicle, 5 mL/kg body weight Arachis oil; Statistical analysis was done by ANOVA followed by post hoc test of significance, Dunnett’s t- test.

*P < 0.05, **P < 0.001, when compared with vehicle control. PECC: petroleum ether extract of aerial parts of C. coromandelina.

Table 2.  Weights of ovary, uterus and pituitary after treatment with petroleum ether extract of aerial parts of C. coromandelina (PECC).

Treatment with dose	Weight of ovaries (mg)	Weight of pituitary (mg)	Weight of uterus (mg)	Carbonic anhydrase activity in uterus (mol/L/s)
Saline	8.4 ± 0.9	1.70 ± 0.07	25.5 ± 1.3	0.33 ± 0.09
(5 mL/kg, i,p.)
Vehicle (Arachis oil)	7.9 ± 0.7	1.52 ± 0.06	27.8 ± 1.1	0.34 ± 0.05
(5 mL/kg, i.p.)
Ethinyl estradiol	3.6 ± 0.7*	0.67 ± 0.06*	12.1 ± 0.4*	1.17 ± 0.09*
(0.03 mg/kg, i.p.)
PECC	4.3 ± 0.3*	0.85 ± 0.04*	13.9 ± 0.7*	0.62 ± 0.04*
(300 mg/kg, i.p.)
PECC	4.1 ± 0.5*	0.79 ± 0.03*	13.0 ± 0.8*	0.72 ± 0.08*
(400 mg/kg, i.p.)
PECC	3.9 ± 0.6*	0.70 ± 0.06*	12.4 ± 0.9*	0.89 ± 0.06*
(500 mg/kg, i.p.)
Ethinyl estradiol	3.5 ± 0.4*	0.65 ± 0.05*	12.0 ± 0.8*	1.52 ± 0.12**
(0.03 mg/kg, i.p.)
+ PECC (300 mg/kg, i.p.)

Results presented as mean ± SEM from 6 animals. Vehicle, 5 mL/kg body weight Arachis oil; statistical analysis was done by ANOVA followed by post hoc test of significance, Dunnett’s t-test. Carbonic anhydrase activity was analyzed by ANOVA followed by Tukey’s test.

*P < 0.05, **P < 0.001 as compared with vehicle control. PECC: petroleum ether extract of aerial parts of C. coromandelina. Enzyme activity is expressed as rate of enzymatic hydration of CO₂/6.9 × 10⁻⁵.